Cloning and structural organization of the gene encoding the mouse glial cell line-derived neurotrophic factor, GDNF

Gene
N MatsushitaK Kiuchi

Abstract

cDNA for glial cell line-derived neurotrophic factor (GDNF) was cloned from mouse neonatal brain by the method of 5'-rapid amplification of cDNA end (5'-RACE), and the sequence of it's 5'-untranslated region (5'-UTR) was determined. The mouse GDNF gene was then isolated from a genomic library and analyzed for its nucleotide sequence. In vitro translation analysis indicated that the second ATG codon in an open reading frame is the translation start point. Structural analysis of the isolated clones showed that the GDNF gene was separated into three exons and the actual translation start point was present in the second exon. RNA blot hybridization analysis indicated that the GDNF mRNA is approximately 4.5 kb long. The transcriptional start site in the GDNF gene was determined and a typical TATA box sequence was found in the promoter region. On the other hand, the gene expression of GDNF in C6 glioma cells was transiently induced by treatment with phorbol myristate acetate (PMA), but not by forskolin.

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Citations

Nov 10, 2000·Biochimica Et Biophysica Acta·M TanakaK Kiuchi
Jun 15, 1999·Brain Research. Molecular Brain Research·P A BaeckerR M Johnson
Jul 16, 2014·Molecular and Cellular Endocrinology·Dante Lamberti, Elena Vicini
Oct 1, 2008·Progress in Neurobiology·Ana SaavedraEmília P Duarte
Feb 26, 2008·Neuroscience Research·Tatsuhide TanakaKazutoshi Kiuchi
Jan 3, 2009·Neurobiology of Disease·Jagdeep K SandhuMarianna Sikorska
Sep 9, 2020·Cell and Tissue Research·Alberto F Cintrón-ColónJohn M Spitsbergen
Jul 2, 2020·RNA Biology·Dingfeng LiQiang Liu
Mar 19, 2020·Metabolic Brain Disease·Shamseddin AhmadiSteven Bradburn

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