PMID: 7029528Aug 1, 1981Paper

Cloning arg3, the gene for ornithine carbamoyltransferase from Saccharomyces cerevisiae: expression in Escherichia coli requires secondary mutations; production of plasmid beta-lactamase in yeast

Proceedings of the National Academy of Sciences of the United States of America
M CrabeelN Glansdorff

Abstract

The yeast arg3 gene, coding for ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase, EC 2.1.3.3), has been cloned on a hybrid pBR322-2-micrometers plasmid. The cloned gene gives a normal regulatory response in yeast. It is not expressed at 35 degrees C when a mutation preventing mRNA export from the nucleus at this temperature is included in the genetic make-up of the carrier strain. In Escherichia coli, no functional expression can be observed from the native yeast arg3 gene. The study of a mutant plasmid (M1) producing low levels of yeast carbamoyltransferase in E. coli has permitted the localization and orientation of arg3 on the plasmid. The mutation involved is a deletion that alters the regulatory response of arg3 in yeast. The plasmid bla gene produces detectable amounts of beta-lactamase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) in yeast: the data provide an estimate of the beta-lactamase activity associated with one exemplar of the plasmid expressing arg3 (0.6 units).

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A HinnenG R Fink
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Sutcliffe
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C P HollenbergH D Royer
Aug 5, 1975·Journal of Molecular Biology·M WolfnerG R Fink

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Citations

Aug 1, 1988·Molecular & General Genetics : MGG·J R PereraA V Boreiko
Jan 1, 1985·Molecular & General Genetics : MGG·S SangsodaY Surdin-Kerjan
Oct 11, 1982·Nucleic Acids Research·D CharlierN Glansdorff
Apr 15, 1983·Biochemical and Biophysical Research Communications·D J BallanceG Turner
Feb 22, 2018·Proteomics·Paige E Erpf, James A Fraser
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·A Coulson
Jun 1, 1989·Molecular and Cellular Biology·M D BaroniL Alberghina

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