Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Yubao CuiWeihong Shi

Abstract

Crude extracts of house dust mites are used clinically for diagnosis and immunotherapy of allergic diseases, including bronchial asthma, perennial rhinitis, and atopic dermatitis. However, crude extracts are complexes with non-allergenic antigens and lack effective concentrations of important allergens, resulting in several side effects. Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae) is one of the predominant sources of dust mite allergens, which has more than 30 groups of allergen. The cDNA coding for the group 5 allergen of D. farinae from China was cloned, sequenced and expressed. According to alignment using the VECTOR NTI 9.0 software, there were eight mismatched nucleotides in five cDNA clones resulting in seven incompatible amino acid residues, suggesting that the Der f 5 allergen might have sequence polymorphism. Bioinformatics analysis revealed that the matured Der f 5 allergen has a molecular mass of 13604.03 Da, a theoretical pI of 5.43 and is probably hydrophobic and cytoplasmic. Similarities in amino acid sequences between Der f 5 and allergens of other domestic mite species, viz. Der p 5, Blo t 5, Sui m 5, and Lep d 5, were 79, 48, 53, and 37%, respectively. Phylogenetic analysis indicated that Der f 5 an...Continue Reading

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Citations

Sep 2, 2014·Parasites & Vectors·Yubao Cui
Jan 13, 2015·Molecular Medicine Reports·Feixiang TengYubao Cui
May 20, 2014·Immunologic Research·Yu-Bao CuiCheng-Bo Zhang
Nov 26, 2013·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Jay PortnoyUNKNOWN Practice Parameter Workgroup
Dec 12, 2018·Protein and Peptide Letters·Jianglong PengYubao Cui
Jun 23, 2015·International Forum of Allergy & Rhinology·Yu-bao CuiCheng-bo Zhang
Feb 5, 2016·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Yu-Bao CuiCheng-Bo Zhang
Feb 3, 2021·International Journal of Molecular Sciences·Tae-Young GilHyo-Jin An
Oct 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hyun-Kyung SongTaesoo Kim
Nov 1, 2018·International Archives of Allergy and Immunology·Sadjia LahianiMoreno Galleni

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Datasets Mentioned

BETA
AY
283283
ABH06352
AAX34051
AAX34060
AY283283
AB195581

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

PROSCAN
CELLO
ProtScale
ProtParam
Mega
ExPaSy
SOPMA
SignalP
Translate Tools
ClustalW2

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