PMID: 7536984Dec 1, 1994Paper

Cloning bovine cytokine cDNA fragments and measuring bovine cytokine mRNA using the reverse transcription-polymerase chain reaction

Veterinary Immunology and Immunopathology
L E HutchinsonS C Olsen

Abstract

Bovine cytokine-specific primers and the reverse transcription-polymerase chain reaction (RT-PCR) were used to clone cDNA fragments that were specific for bovine IL-1 alpha, IL-1 beta, IL-2, and IFN-gamma. Specificity of the cDNA fragments was verified by sequence analysis based on known bovine IL-1 alpha, IL-1 beta, IL-2, and IFN-gamma gene sequences. In addition, RT-PCR was used to monitor cytokine mRNA expression in concanavalin A (Con A) and lipopolysaccharide (LPS)-stimulated bovine peripheral blood mononuclear cells (PBMC), and the results were compared with those obtained by measuring PBMC cytokine secretion using biologic assays. IL-1 activity in LPS-stimulated PBMC cultures was similar at 12 h and 24 h, although the activity decreased by approximately 40% at 48 h. IL-2 and IFN-gamma activity in supernatants of Con A-stimulated PBMC cultures was low at 12 h and reached maximum levels at 48 h. RT-PCR transcript analysis detected an increase in IL-1 alpha, IL-1 beta, IL-2, and IFN-gamma mRNA expression that was usually correlated with the detection of these soluble cytokines by the bioassays. These results indicate that RT-PCR is a sensitive and effective method of obtaining cDNA probes and that this technique can be used...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A B Troutt, A Kelso
Sep 1, 1991·Veterinary Immunology and Immunopathology·J Jensen, R D Schultz
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E S KawasakiF P McCormick
Jun 1, 1988·European Journal of Immunology·M HelleL A Aarden
Aug 27, 1987·The New England Journal of Medicine·I R GarrettG R Mundy
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D P CerrettiP E Baker
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Feb 1, 1981·Journal of Virology·S RubinsteinS Pestka
Feb 1, 1994·Veterinary Immunology and Immunopathology·M G Stevens, S C Olsen
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gregory LeyensBernard Knoops

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Citations

Dec 14, 1999·Journal of Immunological Methods·M Spagnuolo-WeaverG Allan
May 1, 1997·Veterinary Immunology and Immunopathology·R G KeefeJ L Stott
Jun 1, 1997·Veterinary Immunology and Immunopathology·K H NgB M Buddle
Aug 1, 1997·Veterinary Immunology and Immunopathology·A CanalsL C Gasbarre
Feb 14, 1998·Veterinary Parasitology·L C Gasbarre
Nov 7, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·N BoudjellabX Zhao
Oct 6, 2007·The Journal of Veterinary Medical Science·Careen HankangaJun Yasuda
Jul 1, 1997·The Journal of Veterinary Medical Science·H OkadaT J Rosol

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