Cloning, characterization, and activity analysis of a flavonol synthase gene FtFLS1 and its association with flavonoid content in tartary buckwheat

Journal of Agricultural and Food Chemistry
Chenglei LiQi Wu

Abstract

Evidence from in vitro and in vivo studies indicates that rutin, the main flavonoid in tartary buckwheat ( Fagopyrum tataricum ), may have high value for medicine and health. This paper reports the finding of a flavonol synthase (FLS) gene, cloned and characterized from F. tataricum and designated FtFLS1, that is involved in rutin biosynthesis. The FtFLS1 gene was expressed in Escherichia coli BL21(DE3), and the recombinant soluble FtFLS1 protein had a relative molecular mass of 40 kDa. The purified recombinant protein showed, with dihydroquercetin as substrate, total and specific activities of 36.55 × 10(-3) IU and 18.94 × 10(-3) IU/mg, respectively, whereas the total and specific activities were 10.19 × 10(-3) IU and 5.28 × 10(-3) IU/mg, respectively, with dihydrokaempferol. RT-PCR revealed that during F. tataricum florescence there was an organ-specific expression pattern by the FtFLS1 gene, with similar trends in flavonoid content. These observations suggest that FtFLS1 in F. tataricum encodes a functional protein, which might play a key role in rutin biosynthesis.

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Citations

Mar 29, 2014·TheScientificWorldJournal·Yeon Bok KimSang Un Park
Aug 24, 2016·Frontiers in Plant Science·Artur CondeJosé Moutinho-Pereira
Jan 17, 2020·Pakistan Journal of Biological Sciences : PJBS·Aziiz Mardanarian RosdiantoJutti Levita
Feb 9, 2020·International Journal of Molecular Sciences·Sangkyu ParkSun-Hyung Lim
Mar 5, 2014·Journal of Agricultural and Food Chemistry·Xiaohua LiSang Un Park
May 26, 2017·Journal of Agricultural and Food Chemistry·Sangkyu ParkSun-Hyung Lim
Jun 28, 2019·Journal of Agricultural and Food Chemistry·Sangkyu ParkSun-Hyung Lim

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