Cloning, characterization and anion inhibition studies of a γ-carbonic anhydrase from the Antarctic bacterium Colwellia psychrerythraea

Bioorganic & Medicinal Chemistry
Viviana De LucaClemente Capasso

Abstract

We have cloned, purified and characterized the γ-carbonic anhydrase (CA, EC 4.2.1.1) present in the genome of the Antarctic bacterium Colwellia psychrerythraea, which is an obligate psychrophile. The enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons, with the following kinetic parameters: kcat of 6.0×10(5)s(-1) and a kcat/Km of 4.7×10(6)M(-1)×s(-1). This activity was inhibited by the sulfonamide CA inhibitor (CAI) acetazolamide, with a KI of 502nM. A range of anions was also investigated for their inhibitory action against the new enzyme CpsCA. Perchlorate, tetrafluoroborate, fluoride and bromide were not inhibitory, whereas cyanate, thiocyanate, cyanide, hydrogensulfide, carbonate and bicarbonate showed KIs in the range of 1.4-4.4mM. Diethyldithiocarbamate was a better inhibitor (KI of 0.58mM) whereas sulfamide, sulfamate, phenylboronic acid and phenylarsonic acid were the most effective inhibitors detected, with KIs ranging between 8 and 38μM. The present study may shed some more light regarding the role that γ-CAs play in the life cycle of psychrophilic bacteria as the Antarctic one investigated here.

References

Jan 3, 2008·Nature Reviews. Drug Discovery·Claudiu T Supuran
Feb 4, 2009·Biochemistry·Sheridan R MacauleyKevin R Sowers
Dec 8, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·Ramazan DemirdağClaudiu T Supuran
Apr 4, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Claudiu T Supuran
Feb 6, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Claudiu T Supuran
Feb 16, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Fortunato MigliardiniClemente Capasso
May 1, 2013·Expert Opinion on Drug Discovery·Giuseppina De SimoneClaudiu T Supuran
May 1, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Clemente Capasso, Claudiu T Supuran
May 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Anna Di FioreGiuseppina De Simone
Aug 7, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Apr 29, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Clemente Capasso, Claudiu T Supuran
Jun 19, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Jean-Yves Winum, Claudiu T Supuran
Dec 17, 2014·Expert Opinion on Therapeutic Targets·Claudiu T Supuran, Clemente Capasso
Mar 31, 2015·ACS Medicinal Chemistry Letters·Muhammet TancClaudiu T Supuran
Apr 22, 2015·Expert Opinion on Drug Discovery·Claudiu T Supuran, Jean-Yves Winum
Dec 2, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Claudiu T Supuran

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Citations

Jun 30, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Claudiu T Supuran, Clemente Capasso
Feb 24, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Rosa PerfettoClemente Capasso
Mar 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daniela VulloClemente Capasso
May 13, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Rosa PerfettoClemente Capasso
Feb 8, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Andrea AngeliClaudiu T Supuran
Aug 10, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Jun 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sonia Del PreteClemente Capasso
Aug 5, 2020·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Sep 25, 2019·Marine Drugs·Stefano BrunoCinzia Verde
Oct 12, 2019·Bioorganic Chemistry·Alessio NocentiniClaudiu T Supuran
Jan 13, 2021·International Journal of Molecular Sciences·Viviana De LucaClemente Capasso

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