Cloning, characterization, and antigen specificity of T-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients.

Infection and Immunity
A WassenaarF Kievits

Abstract

Chronic periodontitis is characterized by dense infiltrations of B and T lymphocytes within the gingival connective tissue. Distinct anaerobic gram-negative bacteria as well as autoimmunity to collagen have been reported to play a role in the etiology and the pathogenesis of this disease. Here we describe the cloning and characterization of CD4+ and CD8+ T lymphocytes isolated from inflamed gingival tissue obtained from four patients with chronic periodontitis. Clones were raised with phytohemagglutinin and interleukin-2 and tested for proliferation in response to whole-cell antigens of Porphyromonas gingivalis, Prevotella intermedia, Actinobacillus actinomycetemcomitans, human collagen type I, and two bacterial heat shock proteins. CD4+ T-cell clones reactive with collagen type I were obtained from all four patients. Eighty percent of these clones had phenotypes resembling the mouse type 2 T helper (Th) phenotype, i.e., they produced high levels of interleukin-4 and low levels of gamma interferon. No collagen-type-I-reactive CD8+ clones were obtained. Bacterial-antigen-reactive CD4+ and/or CD8+ T-cell clones were also obtained from each patient, and the majority of the clones showed a Th0-like cytokine pattern and produced equ...Continue Reading

References

Jun 1, 1992·The Journal of Experimental Medicine·J T Harty, M J Bevan
Jan 30, 1992·The New England Journal of Medicine·D S RobinsonA B Kay
Jan 1, 1992·Journal of Periodontal Research·O AnusaksathienA E Dolby
Jan 1, 1992·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·A ColasanteP Musiani
Apr 1, 1992·Journal of Periodontology·R J Genco
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P ParronchiS Romagnani
Mar 1, 1991·The Journal of Clinical Investigation·K SelmajC F Brosnan
Mar 1, 1991·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·O Anusaksathien, A E Dolby
May 23, 1985·Journal of Immunological Methods·P H Van der MeideH Schellekens
Jan 1, 1989·Journal of Periodontology·R A ReinhardtW B Kaldahl
May 1, 1987·Journal of Periodontal Research·M A Listgarten
Nov 1, 1988·Journal of Oral Pathology·H Z HirschJ Mestecky
Sep 1, 1985·Infection and Immunity·A J van WinkelhoffJ de Graaff
Mar 1, 1984·Journal of Dental Research·M A TaubmanC L Olson
Jan 1, 1982·International Archives of Allergy and Applied Immunology·W MammoA E Dolby
Nov 1, 1993·Journal of Periodontal Research·G J SeymourM A Taubman
Sep 1, 1994·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·K YamazakiK Hara
Aug 1, 1994·Immunology Today·S D Miller, W J Karpus
Sep 1, 1994·Journal of Periodontology·S S ManhartT M Petro
Mar 1, 1994·Immunology Today·G Opdenakker, J Van Damme
Mar 1, 1994·Immunology Today·D M KemenyD Diaz-Sanchez
Aug 1, 1993·Current Opinion in Immunology·R L Modlin, T B Nutman
Sep 1, 1993·Journal of Periodontal Research·R W Verwer, J E Raber-Durlacher

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Citations

Nov 1, 1996·Journal of Periodontal Research·M D Petit, P Stashenko
Apr 27, 2004·Periodontology 2000·Erica Gemmell, Gregory J Seymour
Feb 9, 2005·Clinical and Diagnostic Laboratory Immunology·Pauline FordGregory Seymour
Jun 24, 2005·Journal of Dental Research·T NakajimaK Yamazaki
Feb 19, 1998·Oral Microbiology and Immunology·K YamazakiK Hara
Feb 5, 1999·Clinical and Experimental Immunology·A WassenaarA Snijders
Mar 27, 1999·Oral Microbiology and Immunology·T NakajimaK Hara
Apr 25, 2000·Journal of Periodontology·C ChampaiboonR Mahanonda
Oct 7, 2000·Journal of Dental Research·O TakeichiM A Taubman
Jan 12, 2007·Periodontology 2000·Erica GemmellGregory J Seymour
Aug 5, 1998·Journal of Periodontal Research·G E SalviS Offenbacher
Nov 21, 1997·Clinical and Experimental Immunology·A WassenaarF Kievits
Jan 18, 2006·Journal of Periodontal Research·R AmanumaK Yamazaki
Apr 27, 2004·Periodontology 2000·Kazuhisa Yamazaki, Takako Nakajima
Apr 17, 2004·Journal of Clinical Periodontology·J R GonzalesJ Meyle
May 3, 2008·Journal of Leukocyte Biology·Cristina Ribeiro CardosoJoão Santana Silva
Aug 2, 2006·Autonomic & Autacoid Pharmacology·M CasanovaE S Borda
Jul 5, 2002·Journal of Dental Research·E GemmellG J Seymour
Oct 24, 2013·BioMed Research International·Rayyan A Kayal
Sep 1, 2005·Journal of Clinical Periodontology·Tord Berglundh, Mauro Donati
Dec 2, 2009·Journal of Dental Research·H LucasD R Haynes
May 10, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A Taubman, T Kawai
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·E GemmellG J Seymour
Jan 1, 1997·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A Mathur, B S Michalowicz
Feb 11, 2000·Archives of Oral Biology·T NakajimaG J Seymour

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