Cloning, characterization, and cellular distribution of rat scavenger receptor class B type I (SRBI) in the ovary

Biochemical and Biophysical Research Communications
T MizutaniK Miyamoto

Abstract

An immediately inducible gene by gonadotropin was isolated from rat ovaries primed with pregnant mare serum gonadotropin (PMSG) by using a subtraction cloning procedure. Homology analysis revealed that the gene is a rat homologue of scavenger receptor class B-I, which was recently identified as a specific receptor for high density lipoprotein (HDL). The structure of rat SRBI was determined by nucleotide sequence analysis of full-length cDNAs for SRBI. Northern blot analysis revealed that rat SRBI mRNA levels were rapidly and strongly increased within 3 h by the injection of PMSG. In situ hybridization study revealed that SRBI mRNA was strongly induced in theca interna cells of immature rat ovary stimulated with 30 IU of PMSG for 6 h. SRBI mRNA expression was also observed in corpora lutea of the adult rat ovary. These findings indicate that expression of SRBI mRNA is restricted to and induced in the ovarian steroidogenic cell types where cholesterol is used as a substrate for synthesis of steroid hormones. Our data strongly suggest that SRBI may play a significant role in the ovarian steroidogenesis by mediating selective uptake of cholesterol from HDL to ovarian theca interna cells or to corpus luteum.

Citations

Nov 6, 2009·Antioxidants & Redox Signaling·Irena LevitanPapasani V Subbaiah
Oct 4, 2006·Reproductive Biology and Endocrinology : RB&E·Miki YoshinoKaoru Miyamoto
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M Krieger
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·B TrigattiM Krieger
Feb 26, 2013·Molecular and Cellular Endocrinology·Yoshitaka ImamichiKaoru Miyamoto
Nov 22, 2005·Molecular and Cellular Endocrinology·Dayami Lopez, Mark P McLean
Jan 25, 2013·International Archives of Allergy and Immunology·R Rühl
Feb 20, 1999·The Journal of Biological Chemistry·A ShiratsuchiY Nakanishi
May 23, 2002·The Journal of Biological Chemistry·Yuki KawasakiYoshinobu Nakanishi
Feb 19, 2000·Current Opinion in Lipidology·M Krieger, K Kozarsky
Apr 29, 2000·Current Opinion in Lipidology·B TrigattiM Krieger
Sep 11, 1999·Current Opinion in Lipidology·D L WilliamsG H Rothblat
May 10, 2000·The Journal of Biological Chemistry·T MizutaniK Miyamoto
Feb 16, 1999·Biochimica Et Biophysica Acta·U P Steinbrecher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.