PMID: 10438791Aug 10, 1999Paper

Cloning, characterization, and expression of a novel gene encoding a reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum

Journal of Bacteriology
J HuangJ Wiegel

Abstract

A novel gene, designated ohb1, which encodes the oxygen-sensitive and biotin-, ATP-, thiamin-, pyridoxal phosphate-, and metal-ion-independent, reversible 4-hydroxybenzoate decarboxylase (4-HOB-DC) from the obligate anaerobe Clostridium hydroxybenzoicum JW/Z-1(T) was sequenced (GenBank accession no. AF128880) and expressed. The 1,440-bp open reading frame (ORF) (ohb1) encodes 480 amino acids. Major properties of the heterologous enzyme (Ohb1) expressed in Escherichia coli DH5alpha were the same as those described for the native 4-HOB-DC (Z. He and J. Wiegel, J. Bacteriol. 178:3539-3543, 1996). The deduced amino acid sequence shows up to 57% identity and up to 74% similarity to hypothetical proteins deduced from ORFs in genomes from bacteria and archaea, suggesting a possible novel gene family.

Citations

Jan 1, 2003·Bioscience, Biotechnology, and Biochemistry·Toyokazu YoshidaToru Nagasawa
Apr 5, 2008·Canadian Journal of Microbiology·Boguslaw LupaJuergen Wiegel
Apr 2, 2008·Annals of the New York Academy of Sciences·Georg Fuchs
Dec 22, 2009·Chemical Society Reviews·Silvia M GlueckKurt Faber
Jul 27, 2002·Annual Review of Microbiology·Jane Gibson, Caroline S Harwood
Oct 13, 2005·Biological Chemistry·Matthias Boll, Georg Fuchs
Oct 13, 2006·Acta Biochimica Et Biophysica Sinica·Jun Liu, Jian-Hua Liu
Oct 4, 2011·Nature Reviews. Microbiology·Georg FuchsJohann Heider
Oct 20, 2009·Archives of Microbiology·Sirko Schmeling, Georg Fuchs
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
Feb 26, 2021·Applied Microbiology and Biotechnology·M Concetta TomeiLorenzo Brusetti
May 7, 2013·Applied and Environmental Microbiology·Natalia JiménezRosario Muñoz
Sep 24, 2021·Journal of Agricultural and Food Chemistry·Xin GaoHui-Min Qin

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