Cloning, characterization, and function of MyD88 in silvery pomfret (Pampus argenteus) in response to bacterial challenge

International Journal of Biological Macromolecules
Quanxin GaoJing Lv

Abstract

Myeloid differentiation factor 88 (MyD88) is a key and universal downstream adapter for most Toll-like receptors (TLRs) and plays an important role in both the innate and adaptive immune response. In this study, the full-length cDNA of MyD88 (PAMyD88) from silvery pomfret (Pampus argenteus) was cloned and characterized. The PAMyD88 cDNA is 1545bp in length and contains an 876bp open reading frames (ORF). Multiple sequence alignment and phylogenetic tree analyzes revealed that the amino acid sequence of PAMyd88 was homologous to a variety of previously MyD88 molecules characterized from other species. The quantitative real-time reverse transcription-PCR analysis showed that the PAMyD88 mRNA was broadly expressed in all examined tissues, with higher levels observed in the immune-relevant organs. The results showed a significant up-regulation of the TLR2 and PAMyD88 transcript levels in response to L. plantarum and C. butyricum and a substantial expression level of TLR4 and PAMyD88 induced by V. anguillarum. Additionally, a challenge with V. anguillarum resulted in significant apoptosis, whereas the L. plantarum and C. butyricum induced only low levels of apoptosis. These data provide insight into the roles of PAMyD88 in the TLR s...Continue Reading

References

Dec 4, 2008·Fish & Shellfish Immunology·Cui-Luan YaoXue-Zhe Han
Apr 14, 2009·Veterinary Immunology and Immunopathology·Alexander ReblHans-Martin Seyfert
May 9, 2009·Developmental and Comparative Immunology·Ingrid SkjaevelandJorunn B Jørgensen
Mar 19, 2011·Developmental and Comparative Immunology·Yniv Palti
Dec 24, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lena NovikovaKlaus Ruckdeschel
Jan 16, 2014·Indian Journal of Microbiology·Quanxin GaoZhaohong Shi
Jan 27, 2016·Applied Biochemistry and Biotechnology·Shudong NiuXianghong Xiao
Feb 11, 2016·Immunology and Cell Biology·Qian Liu, Jeak Ling Ding
Feb 24, 2016·Developmental and Comparative Immunology·Sylvia Brugman
Mar 30, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Song JiangRichard I Tapping
Apr 19, 2017·Biochemical and Biophysical Research Communications·Masaya TakeharaMasahiro Nagahama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis