PMID: 9434123Jan 20, 1998Paper

Cloning, characterization and functional analysis of groESL operon from thermophilic cyanobacterium Synechococcus vulcanus

Biochimica Et Biophysica Acta
N TanakaH Nakamoto

Abstract

Genes encoding 10914 Da and 58267 Da polypeptides homologous to groES and groEL of Escherichia coli were cloned and sequenced from a thermophilic cyanobacterium, Synechococcus vulcanus. The deduced amino acid sequence of the GroEL protein was much more homologous to GroELs of other cyanobacteria which accompany GroES than another GroEL homolog of S. vulcanus (GroEL2) reported previously (M. Furuki, N. Tanaka, T. Hiyama, and H. Nakamoto, Biochim. Biophys. Acta 1294 (1996) 106-110). We designate the gene as groEL1 to distinguish it from the non-operon forming groEL2 gene. A 9-base pair inverted repeat sequence (TTAGCACTC-N9-GAGTGCTAA) was located upstream of the promoter region of groEL1, which was absent in groEL2. Southern blot analysis indicated that only one groESL1 operon was present in the genomic DNA of S. vulcanus. The amount of the bicistronic, 2.3 kb transcript of groESL1 operon increased 30-fold within 30 min upon heat shock. The increase was completely inhibited by chloramphenicol, suggesting the involvement of heat-induced production of a polypeptide. Introduction of the cloned groEL1 gene into a groEL defective mutant of E. coli resulted in the complementation of heat sensitivity, which contrasted with the previous ...Continue Reading

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Citations

Apr 15, 2005·Archives of Microbiology·F Rodríguez-QuiñonesP A Lund
Jun 25, 2003·Biochemical and Biophysical Research Communications· AsadulghaniHitoshi Nakamoto
May 10, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Anna ZorinaDmitry A Los
Mar 6, 2003·Journal of Bacteriology·Karuna P KarunakaranRobert C Brunham
Dec 12, 2001·Biochemical and Biophysical Research Communications·E KovácsL Vígh
Apr 10, 2012·Progress in Lipid Research·Ibolya HorváthLászló Vigh
Apr 9, 2009·FEBS Letters·Ornella CastielliMiguel A De la Rosa
Apr 5, 2013·Biological Reviews of the Cambridge Philosophical Society·Brian HendersonPeter A Lund
Feb 5, 2014·Microbiology·Hema RajaramShree Kumar Apte
May 7, 2009·FEMS Microbiology Reviews·Peter A Lund
Aug 14, 2003·FEBS Letters·Hitoshi NakamotoKouji Kojima
Nov 13, 2010·Bioscience, Biotechnology, and Biochemistry·Saaimatul HuqHitoshi Nakamoto
May 9, 2001·The Journal of Biological Chemistry·H NakamotoN Ishikawa
Oct 1, 2020·Journal of Biochemistry·Tahmina Akter, Hitoshi Nakamoto

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