Cloning, characterization and functional expression of a phospholipase Dalpha cDNA from tomato fruit

Physiologia Plantarum
Bruce D. WhitakerKaren C. Green

Abstract

Phospholipase D (PLD; EC 3.1.4.4) initiates phospholipid (PL) catabolism in plant cells and is also involved in signal transduction and retailoring of membrane PL. Phosphatidic acid (PA), the product of PLD hydrolysis of PL, increases in pericarp tissue during ripening of tomato (Lycopersicon esculentum Mill.) fruit, suggesting that increased PLD activity may be involved in loss of membrane function associated with ripening. However, a recent report showed a decline in soluble PLD activity in both normal and nonripening mutant fruit over the span that encompasses full ripening. To directly assess the role of PLD in tomato ripening, we have initiated a molecular genetic approach. Using a PLDalpha cDNA from castor bean as a probe, a PLDalpha cDNA (LEPLD2) was isolated from a tomato fruit library. It has an open reading frame of 2 421 nucleotides, predicted to encode a polypeptide of 807 amino acids, with a molecular mass of 91.9 kDa. These values are close to those of PLDalphas from 11 plant species and LEPLD2 has >/=73% nucleotide sequence identity with PLDalpha cDNAs from castor bean and tobacco, as well as another tomato cDNA. LEPLD2 transcript was detected in all tissues of the tomato plant by RNA gel-blot analysis. Levels we...Continue Reading

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Citations

Aug 17, 2002·Current Opinion in Plant Biology·Xuemin Wang
Sep 25, 2003·Annual Review of Plant Biology·Harold J G Meijer, Teun Munnik
Feb 2, 2010·Journal of Plant Physiology·A J Moreno-PérezJ J Salas
May 7, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jiang ChangChangxian Yang
Sep 9, 2018·Journal of Separation Science·Jiayu XuYufen Cao
Mar 6, 2002·BMC Genomics·Marek EliásViktor Zárský
Dec 1, 2010·Plant Physiology and Biochemistry : PPB·Krishnaraj Tiwari, Gopinadhan Paliyath

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