PMID: 8444812Mar 1, 1993Paper

Cloning, characterization, and functional expression in Escherichia coli of chaperonin (groESL) genes from the phototrophic sulfur bacterium Chromatium vinosum

Journal of Bacteriology
R G FerreyraA M Viale

Abstract

A recombinant lambda phage which was able to propagate in groE mutants of Escherichia coli was isolated from a Chromatium vinosum genomic DNA library. A 4-kbp SalI DNA fragment, isolated from this phage and subcloned in plasmid vectors, carried the C. vinosum genes that allowed lambda growth in these mutants. Sequencing of this fragment indicated the presence of two open reading frames encoding polypeptides of 97 and 544 amino acids, respectively, which showed high similarity to the molecular chaperones GroES and GroEL, respectively, from several eubacteria and eukaryotic organelles. Expression of the cloned C. vinosum groESL genes in E. coli was greatly enhanced when the cells were transferred to growth temperatures that induce the heat shock response in this host. Coexpression in E. coli of C. vinosum groESL genes and the cloned ribulose bisphosphate carboxylase/oxygenase genes from different phototrophic bacteria resulted in an enhanced assembly of the latter enzymes. These results indicate that the cloned DNA fragment encodes C. vinosum chaperonins, which serve in the assembly process of oligomeric proteins. Phylogenic analysis indicates a close relationship between C. vinosum chaperonins and their homologs present in patho...Continue Reading

References

May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R W Hendrix, L Tsui
May 1, 1992·Journal of Bacteriology·M WetzsteinW Schumann
Jun 1, 1992·Journal of Bacteriology·M Li, S L Wong
Aug 30, 1991·Biochemical and Biophysical Research Communications·H TamadaY Kagawa
Nov 1, 1991·Journal of Bacteriology·P MazodierC J Thompson
Jan 1, 1991·Annual Review of Microbiology·J Zeilstra-RyallsC Georgopoulos
Jun 1, 1987·Microbiological Reviews·C R Woese
Aug 1, 1989·Journal of Bacteriology·W G WeisburgC R Woese
Nov 25, 1988·Nucleic Acids Research·F Corpet
Feb 15, 1988·Archives of Biochemistry and Biophysics·J A Torres-Ruiz, B A McFadden
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D W CowingC A Gross
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K TillyC Georgopoulos
Nov 16, 1990·Science·R J Ellis

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Citations

Mar 21, 1994·FEBS Letters·A M Viale, A K Arakaki
Oct 1, 1995·FEMS Microbiology Reviews·H BahlF Narberhaus
Jun 5, 2003·Microbiology·Dorota Kuchanny-Ardigò, Barbara Lipińska
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Jo Handelsman
Dec 12, 2001·Biochemical and Biophysical Research Communications·E KovácsL Vígh
Mar 1, 1995·Trends in Cell Biology·K W Jeon
Feb 15, 2000·Research in Microbiology·M S Gelfand
Oct 12, 2004·Biochemical and Biophysical Research Communications·Roger GeorgePeter A Lund
Nov 18, 2021·Applied Microbiology and Biotechnology·Anchittha KaewchanaPreekamol Klanrit

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