Cloning, characterization, and molecular expression of gonadotropin receptors in European hake (Merluccius merluccius), a multiple-spawning species

Fish Physiology and Biochemistry
Michela CandelmaOliana Carnevali

Abstract

Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages in the European hake (Merluccius merluccius), a multiple-spawning species. With this aim, fshr and lhr were sequenced, characterized, and their gene expression was quantified in oocytes and in testes at different maturity stages. The deduced amino acid sequences were used to phylogenetic studies and evidenced that both receptors are phylogenetically closed to other gadoid species. The gene expression of both receptors was poorly expressed in primary follicles, increased in vitellogenic follicles and to later decrease in hydrated oocytes. In testis, highest levels of lhr were detected during spermiation, while levels of fshr were constant. For the first time, a histological analysis was performed in European hake testes showing an unrestricted lobular testis. To better elucidate the mechanisms involved in the oogenesis of the European hake, the expression of estrogen receptor and cyp19a was also investigated displayi...Continue Reading

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Datasets Mentioned

BETA
ABD62885
ABD62886
AAV48629
AAT01412
BAB16106
AAR84280
AAR84281
BAM33585
CPR30244
ACF35638

Methods Mentioned

BETA
PCRs
light microscopy

Software Mentioned

BLAST Suite
iQ5
MEGA6
Prism
MEGA
Genex
Basic Local Alignment Search Tool ( BLAST
SMART
Expasy Translate
ClustalO

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