PMID: 8955666Oct 1, 1996Paper

Cloning, DNA sequence, and alternative splicing of opossum amelogenin mRNAs

Journal of Dental Research
C C HuJ P Simmer

Abstract

The enamel layer that covers the surfaces of teeth is thickest and most highly mineralized in mammals. The durability of mammalian enamel may have allowed for selection against the lifelong replacement of teeth that is observed in other vertebrates. Variation in enamel structure among animals is thought to be the result of evolutionary changes in the constituents of the developing enamel matrix. In placental mammals, the principal component of this matrix is amelogenin. We have determined the complete primary structures of two opossum amelogenins through a combination of protein sequencing, cloning, and DNA sequencing. RNA messages were cloned that encode 202- and 57-residue amelogenins, which are presumed to be expressed from the same gene but differ due to alternative splicing of identical pre-mRNAs. Edman degradation of the larger amelogenin ran for 42 cycles and yielded the sequence: IPLPPHPGHPGYINFS YEVLTPLKWYQSMMRQQYPSYGYEPM. The derived 202-residue amelogenin, assuming that serine 16 is phosphorylated, has an isotope-averaged molecular mass of 23,023.75 Daltons and a pI of 6.2. This is the largest amelogenin yet characterized. The increase in length is due to the presence of a 30-residue tandem repeat of QP(I/M) in exon ...Continue Reading

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Citations

Sep 6, 2003·Calcified Tissue International·E ChenC W Gibson
Oct 16, 2009·Journal of Dental Research·Y DingJ C-C Hu
Apr 25, 2008·Journal of Dental Research·K TanimotoW Li
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·S ToyosawaJ Klein
Dec 20, 2002·Connective Tissue Research·C C HuJ P Simmer
Dec 20, 2002·Connective Tissue Research·James P Simmer, Jan C C Hu
Nov 4, 2000·Connective Tissue Research·C C HuM MacDougall
Aug 12, 1999·Journal of Structural Biology·A G FinchamJ P Simmer
May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C E Smith
Dec 6, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J C-C Hu, Y Yamakoshi
Dec 10, 1998·Journal of Clinical Periodontology·B L DonaG A van der Weijden
Sep 29, 2011·Journal of Dental Research·J-Y SireP K Denbesten
May 6, 2006·European Journal of Oral Sciences·T G H DiekwischX Luan
Feb 8, 2005·Journal of Molecular Evolution·Sidney DelgadoJean-Yves Sire
Mar 4, 2005·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xinping WangThomas G H Diekwisch
Mar 1, 2006·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xinping WangThomas G H Diekwisch
Jul 14, 2007·Cells, Tissues, Organs·Mineyo IwaseNaoyuki Takahata

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