Cloning, expression and characterization of a cell wall surface protein, 6-phosphogluconate-dehydrogenase, of Streptococcus suis serotype 2

Veterinary Microbiology
Chen TanHuanchun Chen

Abstract

Streptococcus suis type 2 is a pathogen responsible for diverse diseases in both pigs and humans. In order to understand the pathogenesis of the S. suis type 2 infection, the gene encoding a cell surface protein, 6-phosphogluconate-dehydrogenase (6PGD) of S. suis type 2 was cloned and sequenced, and recombinant 6PGD protein (r6PGD) was produced in a prokaryotic expression system. Sequence analysis of the cloned 6 pdg gene showed 82% similarity with Streptococcus pneumoniae 6 pdg at the nucleic acid level. Western blotting using r6PGD-specific antiserum confirmed the cell surface location of the 6PGD protein of S. suis type 2. The role of 6PGD in S. suis type 2 pathogenesis as an adhesin and its immunogenicity in mice was further investigated. The results showed that the recombinant protein interfered with the adhesion of S. suis type 2 to Hep2 and HeLa cells by 72% and 66%, respectively. Immunization of CD-1 mice with r6PGD increased the protective efficacy by 80% following intraperitoneal administration of a lethal dose of S. suis type 2. Immunization of CD-1 mice with r6PGD elicited a significant protective immune response, which demonstrated the importance of 6PGD to bacterial pathogenesis. Identification and characterizatio...Continue Reading

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Citations

Apr 19, 2011·Nihon saikingaku zasshi. Japanese journal of bacteriology·Daisuke Takamatsu
Feb 2, 2013·Applied Microbiology and Biotechnology·Jian WangShuo Wang
Oct 16, 2015·Expert Review of Vaccines·Mariela Segura
Oct 3, 2013·The Veterinary Journal·Mar Costa-Hurtado, Virginia Aragon
Jun 30, 2009·Animal Health Research Reviews·Christoph Georg Baums, Peter Valentin-Weigand
Mar 4, 2017·Archives of Microbiology·Niurka MenesesSergio Encarnación
Oct 4, 2018·Frontiers in Cellular and Infection Microbiology·Haodan ZhuKongwang He

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