Cloning, expression and characterization of l-asparaginase from Withania somnifera L. for large scale production.

3 Biotech
Vishal P OzaR B Subramanian

Abstract

l-Asparaginase (E.C. 3.5.1.1) is used as a therapeutic agent in the treatment of acute childhood lymphoblastic leukemia. It is found in a variety of organisms such as microbes, plants and mammals. In plants, l-asparaginase enzymes are required to catalyze the release of ammonia from asparagine, which is the main nitrogen-relocation molecule in these organisms. An Indian medicinal plant, Withania somnifera was reported as a novel source of l-asparaginase. l-Asparaginase from W. somnifera was cloned and overexpressed in E. coli. The enzymatic properties of the recombinant enzyme were investigated and the kinetic parameters (K(m), k(cat)) for a number of substrates were determined. The kinetic parameters of selected substrates were determined at various pH and the pH- and temperature-dependence profiles were analyzed. WA gene successfully cloned into E. coli BL21 (DE3) showed high asparaginase activity with a specific activity of 17.3 IU/mg protein.

References

Dec 26, 2001·Acta Crystallographica. Section D, Biological Crystallography·Maciej KozakMariusz Jaskólski
Sep 23, 2003·Advanced Drug Delivery Reviews·Michael L Graham
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Douglas S HawkinsVassilios I Avramis
Jun 14, 2005·Journal of Biotechnology·Georgia A Kotzia, Nikolaos E Labrou
May 27, 2006·Journal of Molecular Biology·Karolina MichalskaMariusz Jaskolski
Sep 21, 2006·Journal of Biotechnology·Georgia A Kotzia, Nikolaos E Labrou
Oct 3, 2006·Critical Reviews in Oncology/hematology·Umesh K NartaWamik Azmi
Feb 10, 2009·Journal of Integrative Plant Biology·Vishal P OzaR B Subramanian
May 19, 2009·Applied Biochemistry and Biotechnology·Vishal P OzaR B Subramanian

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Citations

Sep 6, 2013·Journal of Agricultural and Food Chemistry·Mingmei JiaZhiming Rao
Jan 19, 2020·Biotechnology and Applied Biochemistry·Subhash ChandGirish Sharma
Aug 13, 2017·Applied Microbiology and Biotechnology·Ali GhasemiBahareh Dabirmanesh

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Datasets Mentioned

BETA
Z34884

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
GraFit

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