Cloning, expression and characterization of the catalase-peroxidase (KatG) gene from a fast-growing Mycobacterium sp. strain JC1 DSM 3803

Journal of Biochemistry
Hyun-Il LeeYoung-Tae Ro

Abstract

The gene encoding a catalase-peroxidase (KatG) was cloned from chromosomal DNA of a fast-growing Mycobacterium sp. strain JC1 DSM 3803. The nucleotide sequence of a 5.7 kb EcoRI fragment containing the katG and its flanking regions was determined. The fragment (5,706 bps) contained two complete open reading frames (ORFs) encoding putative ferric uptake regulator A (FurA) and KatG proteins. The cloned gene, katG, had an ORF of 2241 nt, encoding a protein with calculated molecular mass of 81,748 Da. The furA was located in the upstream of the katG with the same transcriptional direction and there was a 38 bp gap space between them. The deduced KatG and FurA protein sequences showed significant homologies to KatG2 and Fur2 of Mycobacterium smegmatis and clustered with other mycobacterial KatG and Fur-like proteins in phylogenetic trees, respectively. The recombinant KatG overproduced in Escherichia coli was nearly indistinguishable from the native JC1 catalase-peroxidase in enzymatic properties and also possessed the resistance to organic solvents, indicating that the cloned katG truly encodes the Mycobacterium sp. JC1 catalase-peroxidase. Difference spectroscopy revealed Mn(II) binding near the haem of the KatG. Transcript analys...Continue Reading

References

Sep 8, 1978·Science·I Fridovich
Sep 22, 1995·The Journal of Biological Chemistry·J A MarcinkevicieneJ S Blanchard
Oct 1, 1994·The Journal of Applied Bacteriology·D N Love, J Redwin
Apr 4, 1997·The Journal of Biological Chemistry·R S Magliozzo, J A Marcinkeviciene
Jan 25, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S Ramaswamy, J M Musser
Apr 27, 2000·Trends in Genetics : TIG·D M Faguy, W F Doolittle
Jun 20, 2000·FEBS Letters·J A CaleraF Leal
Jan 11, 2000·Nucleic Acids Research·J D PetersonO White
Jan 4, 2001·The Korean Journal of Parasitology·D N Onah, Y Nawa
Mar 17, 2001·Molecular Microbiology·T C ZahrtV Deretic
Mar 21, 2001·FEBS Letters·M ZámockýC Obinger
Dec 18, 2002·Journal of Bacteriology·Sae W ParkYoung M Kim
Mar 12, 2003·Journal of Molecular Biology·Xavi CarpenaIgnacio Fita
Sep 2, 2003·Journal of Bacteriology·Claudia SalaDaniela Ghisotti
Jul 3, 2004·The Journal of Biological Chemistry·Thomas BertrandKatherine A Brown
Jul 3, 2004·The Journal of Biological Chemistry·Roberta PierattelliKatherine A Brown
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Mar 7, 2006·Journal of Inorganic Biochemistry·Giulietta SmulevichChristian Obinger

❮ Previous
Next ❯

Citations

Mar 28, 2016·Journal of Industrial Microbiology & Biotechnology·Tuyishime PhilibertNiyomukiza Samuel
Feb 27, 2015·Biological Chemistry·Melanie Hillion, Haike Antelmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.