Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis

Journal of Biotechnology
Jiquan ZhangJianhai Xiang

Abstract

Ferritin, the iron storage protein, plays a key role in iron metabolism. A cDNA encoding ferritin (FcFer) was cloned from hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The predicted protein contains 170 amino acid residues with a predicted molecular weight (MW) about 19, 422.89 Da and theoretical isoelectric point (PI) of 4.73. Amino acid alignment of FcFer revealed 97% homology with Litopenaeus vannamei ferritin. Results of the RT-PCR showed that the expression of FcFer mRNA was up-regulated after shrimp was challenged with either white spot syndrome virus (WSSV) or heavy metal ions (Zn2+ and Cu2+) in the laboratory. A fusion protein containing FcFer was produced and the purified recombinant protein exhibited similar function of iron uptake in vitro. The result of in-gel digestion and identification using LC-ESI-MS showed that two peptide fragments (-DDVALPGFAK- and -LLEDEYLEEQVDSIKK-) of the recombinant protein were identical to the corresponding sequence of L. vannamei ferritin. The recombinant FcFer protein will be proved useful for study on the structure and function of ferritin in F. chinensis.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1972·The Biochemical Journal·I G MacaraP M Harrison
Jul 31, 1996·Biochimica Et Biophysica Acta·P M Harrison, P Arosio
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei

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Citations

Jun 15, 2007·Biotechnology Letters·Jiquan Zhang, Yuying Sun
Nov 21, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jie-Lan JiangChao-Shu Zeng
May 15, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bo ZhuHe-Qing Huang
Dec 8, 2011·Fish & Shellfish Immunology·Juan LiGuofan Zhang
Mar 3, 2011·Fish & Shellfish Immunology·Chenghua LiTaiwu Li
Jan 5, 2010·Fish & Shellfish Immunology·Pengfei KongLinsheng Song
May 5, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiaomei WangJianhai Xiang
Dec 24, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Ping WuSongnian Hu
May 24, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gao-Feng QiuPing Liu
Mar 6, 2007·Fish & Shellfish Immunology·Alejandra Clavero-SalasGloria Yepiz-Plascencia
Jul 23, 2013·Fish & Shellfish Immunology·Yanhong ChiShihao Li
Jul 22, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Chenglong WuZhiguo Liufu
Aug 14, 2018·Protein Science : a Publication of the Protein Society·Taro MasudaBunzo Mikami
Oct 12, 2012·Molecular Biology Reports·Jiquan ZhangJianhai Xiang
Oct 23, 2020·Food Science and Biotechnology·Selvakumari UlagesanYoun-Hee Choi
Apr 17, 2021·Critical Reviews in Food Science and Nutrition·Han LiMing Du
May 7, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Min LiShicui Zhang
May 29, 2012·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Hui QiaoYiwei Xiong

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