Cloning, expression and localization of DacaCSP2 and DacaCSP3 during different reproductive stages in Daphnia carinata

Gene
Haixia LiYun-Long Zhao

Abstract

Daphnia carinata are unique freshwater crustaceans that undergo both sexual and asexual reproduction, depending on environmental factors. While the molecular mechanism behind the reproductive transformation has been unknown, chemosensory proteins (CSPs) may be involved. We have cloned the cDNA sequences of two CSP genes from D. carinata using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). The full-length cDNA of DacaCSP2 (GenBank accession no: KM624608) was 632 bp, with an ORF (open reading frame) of 330 bp encoding a 12.02 kDa protein; and the full-length cDNA of DacaCSP3 (GenBank accession no: KM624609) was 935 bp, with an ORF of 342 bp encoding a 12.78kDa protein. Both CSPs encoded an N-terminal signal peptide, four conserved cysteines, an OS-D superfamily domain, a 2Fe-2S ferredoxin domain, an anaphylatoxin domain and an EGF-like domain. DaCaCSP2 and DaCaCSP3 proteins were most closely related to CSPs from Daphnia pulex and were more distantly related to CSPs from other insects. Using quantitative PCR, we found expression levels of DaCaCSP2 and DaCaCSP3 mRNA were highest in sexual females, followed by parthenogenetic females, and lowest in males. The expression levels of DaCaCSP2 and DaCa...Continue Reading

References

Jan 1, 1996·Insect Biochemistry and Molecular Biology·A TucciniP Pelosi
Sep 1, 1996·Insect Biochemistry and Molecular Biology·M MameliP Pelosi
Mar 25, 2000·Insect Biochemistry and Molecular Biology·J F PicimbonJ Krieger
Sep 16, 2000·Insect Biochemistry and Molecular Biology·S MarcheseP Pelosi
Jan 10, 2002·Nature·V Alekseev, W Lampert
Nov 27, 2002·Die Naturwissenschaften·Yuko IshidaWalter S Leal
Mar 26, 2003·Insect Molecular Biology·L BanPaolo Pelosi
Mar 1, 2005·Proceedings. Biological Sciences·Jerome C RegierRobert E Kambic
Dec 19, 2006·Insect Biochemistry and Molecular Biology·Sylvain ForêtRyszard Maleszka
May 11, 2013·Yi chuan = Hereditas·Chun-Xia Zhang, Feng Liu
Oct 8, 2013·International Journal of Biological Macromolecules·Xin YiGuoHua Zhong
Mar 13, 2014·Development Genes and Evolution·Ajing LiuYunlong Zhao

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Citations

Feb 2, 2021·Molecular Ecology·Tiffany A Chin, Melania E Cristescu

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