Cloning, expression, and mapping of CKAPI, which encodes a putative cytoskeleton-associated protein containing a CAP-GLY domain

Cytogenetics and Cell Genetics
Tsuyoshi WatanabeY Hirai

Abstract

From a human fetal-brain cDNA library we isolated a novel cDNA clone encoding a protein containing a CAP-GLY domain that is highly conserved among several cytoskeleton-associated proteins. The CAP-GLY domain is thought to be essential for their association with microtubules. The cDNA, designated CKAPI (for cytoskeleton-associated protein I, glycine motif) contained an open reading frame of 579 nucleotides encoding 193 amino acids. Northern-blot analysis revealed expression of three transcripts, 1.0, 3.4, and 4.6 kb in size, in all tissues examined. The 1.0-kb transcript was significantly higher in brain and heart than in other tissues. This gene was mapped by FISH to chromosome bands 19q13.11-->q13.12.

Citations

Mar 20, 2014·International Journal of Biological Sciences·Siriluck PonsuksiliKlaus Wimmers
Aug 1, 2006·Biochemical and Biophysical Research Communications·Sang-Min JeonJoobae Park
Dec 23, 2006·Experimental Cell Research·D KortazarJ C Zabala
Sep 11, 2002·The Journal of Biological Chemistry·Songlin LiMing Luo
Aug 24, 2001·Journal of Molecular Biology·E Sprinzak, H Margalit

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