Cloning, expression, crystallization and preliminary X-ray analysis of a putative multiple antibiotic resistance repressor protein (MarR) from Xanthomonas campestris

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Zhi-Le TuShan-Ho Chou

Abstract

The multiple antibiotic resistance operon (marRAB) is a member of the multidrug-resistance system. When induced, this operon enhances resistance of bacteria to a variety of medically important antibiotics, causing a serious global health problem. MarR is a marR-encoded protein that represses the transcription of the marRAB operon. Through binding with salicylate and certain antibiotics, however, MarR can derepress and activate the marRAB operon. In this report, the cloning, expression, crystallization and preliminary X-ray analysis of XC1739, a putative MarR repressor protein present in the Xanthomonas campestris pv. campestris, a Gram-negative bacterium causing major worldwide disease of cruciferous crops, are described. The XC1739 crystals diffracted to a resolution of at least 1.8 A. They are orthorhombic and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 39.5, b = 54.2 and c = 139.5 A, respectively. They contain two molecules in the asymmetric unit from calculation of the self-rotation function.

References

Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·R G Martin, J L Rosner
May 15, 1996·FEMS Microbiology Letters·A M George
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Sep 28, 1999·Trends in Microbiology·M N Alekshun, S B Levy
Dec 11, 1999·Nucleic Acids Research·A BatemanE L Sonnhammer
Dec 19, 2000·Yeast·J M Dow, M J Daniels
Feb 2, 2002·Nature·M SalanoubatC A Boucher
May 30, 2002·The Journal of Biological Chemistry·Daniel LimNatalie C J Strynadka
Jun 19, 2002·Protein Science : a Publication of the Protein Society·Yan-Ping ShihTing-Fang Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.