Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens

Acta Crystallographica. Section F, Structural Biology Communications
Fuzhou YeYong Gui Gao

Abstract

Two proteins, SghA and SghR, which were recently identified and characterized as novel bacterial virulence factors regulating the infection of plant hosts by Agrobacterium, were cloned, overexpressed and purified with high yield. Both SghA and SghR form dimers in solution. The purified SghA and SghR were crystallized and the crystals diffracted to 1.9 and 2.1 Å resolution, respectively. Data were collected and processed, and the crystallographic parameters were within acceptable ranges. These results will help in the determination of their structures in order to uncover the molecular mechanism of how these two proteins together control the release of plant defence signals against agrobacteria during pathogen-host interaction.

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Citations

Oct 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chao WangLian-Hui Zhang
Jul 12, 2020·The Journal of Biological Chemistry·Fuzhou YeYong-Gui Gao

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Methods Mentioned

BETA
genetic modification
PCR
gel-filtration

Software Mentioned

BLAST
XDS

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