Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Mark J KraschnefskiHelen Blanchard

Abstract

Rotaviruses exhibit host-specificity and the first crystallographic information on a rotavirus strain that infects humans is reported here. Recognition and attachment to host cells, leading to invasion and infection, is critically linked to the function of the outer capsid spike protein of the rotavirus particle. In some strains the VP8* component of the spike protein is implicated in recognition and binding of sialic-acid-containing cell-surface carbohydrates, thereby enabling infection by the virus. The cloning, expression, purification, crystallization and initial X-ray diffraction analysis of the VP8* core from human Wa rotavirus is reported. Two crystal forms (trigonal P3(2)21 and monoclinic P2(1)) have been obtained and X-ray diffraction data have been collected, enabling determination of the VP8*(64-223) structure by molecular replacement.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
May 11, 1984·Nucleic Acids Research·M L Dyall-Smith, I H Holmes
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·B S CoulsonD J Lee
Sep 23, 2000·Journal of Virology·C A GuerreroC F Arias
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Stacy A ScottHelen Blanchard

❮ Previous
Next ❯

Citations

Feb 6, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yang-De ZhangYi-Feng Pan
Jun 10, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Xing YuHelen Blanchard
Aug 19, 2008·Current Opinion in Structural Biology·Mark von Itzstein
Jul 6, 2014·Current Opinion in Virology·David F Smith, Richard D Cummings
Jan 6, 2015·Nature Communications·Raphael BöhmThomas Haselhorst
Feb 3, 2021·Biotechnology and Bioengineering·Moo Sun HongRichard D Braatz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.