Abstract
The Escherichia coli gene encoding the transcription cleavage factor GreB and the Thermus thermophilus gene encoding the anti-GreA transcription factor Gfh1 were cloned and expressed and the purified proteins were crystallized by the sitting-drop vapor-diffusion technique. The GreB and Gfh1 crystals, which were improved by macroseeding, belong to space group P4(1)2(1)2 (or P4(3)2(1)2), with unit-cell parameters a = b = 148, c = 115.2 A and a = b = 59.3, c = 218.9 A, respectively. Complete diffraction data sets were collected for the GreB and Gfh1 crystals to 2.6 and 2.8 A resolution, respectively. Crystals of the selenomethionine proteins were obtained by microseeding using the native protein crystals and diffract as well as the native ones. The structure determination of these proteins is now in progress.
References
Feb 16, 1995·Nature·C E StebbinsS A Darst
Nov 5, 1993·Science·D A ErieP H von Hippel
Dec 16, 2000·The EMBO Journal·F ToulméA R Rahmouni
Feb 13, 2001·Molecular Cell·M T Marr, J W Roberts
Oct 19, 2001·The Journal of Biological Chemistry·Brian P HoganDorothy A Erie
Sep 6, 2002·Biochimica Et Biophysica Acta·Rachel N Fish, Caroline M Kane
Aug 14, 2003·Cell·Ronald C ConawayJoan Weliky Conaway
Aug 14, 2003·Cell·Natacha OpalkaSeth A Darst
Aug 14, 2003·Cell·Hubert KettenbergerPatrick Cramer
Nov 25, 2003·The EMBO Journal·Oleg LaptenkoSergei Borukhov
Nov 25, 2003·Nature·Joshua W ShaevitzSteven M Block
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina SosunovaArkady Mustaev
Jan 10, 2004·Methods in Enzymology·Oleg Laptenko, Sergei Borukhov
Apr 28, 2004·Cell·Irina ArtsimovitchDmitry G Vassylyev
Aug 6, 2004·Cell·Anna PerederinaDmitry G Vassylyev
Apr 6, 2005·Molecular Cell·Maria L KireevaMikhail Kashlev
Jul 26, 2005·Molecular Cell·Brigitte W TrautingerRobert G Lloyd
Citations
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Eric J Strobel, Jeffrey W Roberts
Oct 6, 2007·EMBO Reports·Marina N VassylyevaDmitry G Vassylyev
May 18, 2016·ACS Synthetic Biology·Lea L de MaddalenaSebastian J Maerkl
Oct 5, 2016·ELife·Matti Turtola, Georgiy A Belogurov
Feb 20, 2014·Nucleic Acids Research·Eric J Strobel, Jeffrey W Roberts
Sep 10, 2019·Nucleic Acids Research·Ranjit K PrajapatiGeorgiy A Belogurov
Apr 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Flint Stevenson-JonesNikolay Zenkin