PMID: 9532795Apr 9, 1998Paper

Cloning genomic DNA encoding apple polyphenol oxidase and comparison of the gene product in Escherichia coli and in apple

Bioscience, Biotechnology, and Biochemistry
Miyoshi HarutaS Homma

Abstract

Two PCR-amplified genomic DNA fragments encoding apple (cv. Fuji) polyphenol oxidase (PPO) were cloned and sequenced. A comparison of genomic DNA with cDNAs revealed that the PPOs lacked introns. Both PPO DNAs appear to encode a 66-kDa precursor protein consisting of a 56-kDa mature protein and a N-terminal transit peptide of 10-kDa N-terminal transit peptide. Apple PPO DNA was expressed in Escherichia coli, and the gene product (56 kDa) without a transit peptide was immunochemically detected and was the same size (ca. 65 kDa) as the main PPO of apple fruit by SDS-PAGE.

References

Oct 1, 1992·Plant Molecular Biology·J W CaryW H Flurkey
Jan 1, 1993·Plant Molecular Biology·M D HuntJ C Steffens
Jan 1, 1995·Plant Molecular Biology·P K BossG S Ross
Oct 1, 1994·Plant Molecular Biology·I B Dry, S P Robinson
Jul 10, 1998·Molecular and Biochemical Parasitology·C MarañónM C López
Feb 1, 1992·Plant Physiology·C GanesaW H Flurkey
Jan 1, 1995·IEEE Transactions on Medical Imaging·F J Beekman, M A Viergever
Jan 1, 1995·Journal of Back and Musculoskeletal Rehabilitation·R P Wilder

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Citations

May 21, 2011·Bioscience, Biotechnology, and Biochemistry·Kanako AmakiMasatsune Murata
Nov 10, 2013·PloS One·Mareike E Dirks-HofmeisterBruno M Moerschbacher
Jan 11, 2007·Molekuliarnaia biologiia·Zhihua LiaoXiaozhong Lan
Jul 28, 2010·Journal of the Science of Food and Agriculture·Jing-wei LiuDe-jiang Ni
Oct 26, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Piyada ThipyapongJutharat Attajarusit
Feb 6, 2020·Scientific Reports·Felix PanisAnnette Rompel
Nov 25, 2020·Phytochemistry·Jin Zhang, Xiaoling Sun
Nov 23, 2000·Journal of Agricultural and Food Chemistry·M MurataY Itoh

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