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Cloning, isolation and characterization of the Thermotoga maritima KDPG aldolase

Bioorganic & Medicinal Chemistry

Jan 30, 2002

Jennifer S GriffithsE J Toone

PMID: 11814840

Abstract

The Thermotoga maritima aldolase gene has been cloned into a T7 expression vector and overexpressed in Escherichia coli. The preparation yields 470 UL(-1) of enzyme at a specific activity of 9.4 U mg(-1). During retroaldol cleavage of KDPG, the enzyme shows a k(cat) that decreases with ...read more

Mentioned in this Paper

Pyridines
Alkalescens-Dispar Group
Enzymes, antithrombotic
expression vector
Phospho-2-keto-3-deoxy-gluconate aldolase
Aldehyde-Lyases
Pyruvate Measurement
Cytokinesis of the Fertilized Ovum
Pyruvate
Pyridine
Paper Details
References
  • References12
  • Citations12
12
  • References12
  • Citations12
12

Related Papers

Proceedings of the National Academy of Sciences of the United States of America

Covalent intermediate trapped in 2-keto-3-deoxy-6- phosphogluconate (KDPG) aldolase structure at 1.95-A resolution

Proceedings of the National Academy of Sciences of the United States of AmericaMarch 29, 2001
J AllardJ Sygusch
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Cloning, isolation and characterization of the Thermotoga maritima KDPG aldolase

Bioorganic & Medicinal Chemistry

Jan 30, 2002

Jennifer S GriffithsE J Toone

PMID: 11814840

DOI:

Abstract

The Thermotoga maritima aldolase gene has been cloned into a T7 expression vector and overexpressed in Escherichia coli. The preparation yields 470 UL(-1) of enzyme at a specific activity of 9.4 U mg(-1). During retroaldol cleavage of KDPG, the enzyme shows a k(cat) that decreases with ...read more

Mentioned in this Paper

Pyridines
Alkalescens-Dispar Group
Enzymes, antithrombotic
expression vector
Phospho-2-keto-3-deoxy-gluconate aldolase
Aldehyde-Lyases
Pyruvate Measurement
Cytokinesis of the Fertilized Ovum
Pyruvate
Pyridine

Related Papers

Proceedings of the National Academy of Sciences of the United States of America

Covalent intermediate trapped in 2-keto-3-deoxy-6- phosphogluconate (KDPG) aldolase structure at 1.95-A resolution

Proceedings of the National Academy of Sciences of the United States of AmericaMarch 29, 2001
J AllardJ Sygusch
Paper Details
References
  • References12
  • Citations12
12
  • References12
  • Citations12
12
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