Cloning, localization and bioinformatics analysis of a gene encoding an odorant-binding protein (OBP) in Anoplophora glabripennis (Motschulsky)

Invertebrate Neuroscience : in
Yu LiAihua Yan

Abstract

Anoplophora glabripennis (Motschulsky) has an advanced and complicated olfactory system to identify hosts, mates and spawning locations, and odorant-binding proteins (OBPs) play a key role by binding to volatile materials from different hosts. The full-length cDNA sequence of an OBP, AglaOBP, was cloned by RACE from an antenna cDNA library, and the protein structure and function were predicted by bioinformatics analysis. Gene temporal and spatial expression was detected by real-time qPCR. AglaOBP had distinctive sequence, location and expression profiles compared with other OBPs of A. glabripennis, as it was found in different tissues, and the highest expression was in the elytrums. The possible physiological functions of this OBP were discussed. These findings help elucidate the physiology of this pest and provide a new potential target for pest control.

References

Jul 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P Pelosi, R Maida
Mar 15, 2005·Insect Biochemistry and Molecular Biology·M CalvelloP Pelosi
Sep 10, 1981·Nature·R G Vogt, L M Riddiford
May 27, 2008·Biochemical and Biophysical Research Communications·Sha LiPaolo Pelosi
Aug 8, 2014·Archives of Insect Biochemistry and Physiology·Qian JuFei Li
Jun 11, 2016·Scientific Reports·Zheng PingDejun Shen
Dec 6, 2016·Current Biology : CB·Ruibin ZhangGuirong Wang

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Datasets Mentioned

BETA
KX660670
KY039580.1

Methods Mentioned

BETA
PCR

Software Mentioned

BLASTn
LOMETS
SignalP
BLASTx
ORF Finder
TASSER
DNAMAN
ExPASy
PyMOL
MEGA

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