Cloning of a cDNA associated with acute and chronic hepatitis C infection generated from patients serum RNA

Gastroenterologia Japonica
T ArimaT Tsuji

Abstract

In order to clone hepatitis C (blood-borne non-A, non-B hepatitis) virus, lambda gt11-cDNA library was constructed from RNA extracted from 100 liters serum collected from 1,047 donors with elevated ALT levels and negative for hepatitis B virus-DNA. The library was immunoscreened on Y1090 cells with pooled serum obtained from patients with acute hepatitis C or chronic hepatitis C. By screening 29 clones specific for Japanese hepatitis C infection were isolated. The specificity of these clones for hepatitis C infection was determined by panels constructed in 3 laboratories. Of these, 12 clones were specific for American hepatitis C infection as well. The nucleotide sequence (201 bp) of one of them was determined to be unique compared to known human viruses including hepatitis A virus, hepatitis B virus and hepatitis D virus. Southern blot analysis showed the absence of the sequence of the human genome in the clone. The predicted amino acid sequence is rich in residues of lysine, arginine, glutamic acid and asparagine, while lacking leucine, cysteine and methionine.

References

Jul 1, 1979·Annals of Internal Medicine·M BermanE A Jones
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki
Dec 1, 1981·The Journal of Infectious Diseases·S M FeinstoneR H Purcell

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Citations

Jul 1, 1991·Journal of Gastroenterology and Hepatology·K Kiyosawa, S Furuta
Aug 1, 1990·Gastroenterologia Japonica·H ShimomuraT Tsuji
Jul 1, 1994·FEMS Microbiology Reviews·T Arima
Jan 1, 1994·Digestive Diseases and Sciences·K HinoS Iino
Apr 1, 1990·Gastroenterologia Japonica·T ArimaT Tsuji
Aug 1, 1991·Postgraduate Medical Journal·P M HarrisonR Williams
May 1, 1993·Gastroenterologia Japonica·S M AkbarY Ohta
Mar 1, 1994·Postgraduate Medical Journal·S D Ryder, R Williams
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N KawarabayashiH Mochizuki
Mar 1, 1994·Current Microbiology·Z Qi, T Arima

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