Cloning of a cDNA from Arabidopsis thaliana homologous to the human XPB gene

Gene
D T RibeiroCarlos F M Menck

Abstract

The human gene XPB, defective in xeroderma pigmentosum patients complementation group B, encodes a DNA helicase involved in several DNA metabolic pathways, including DNA repair and transcription. The high conservation of this gene has allowed the cloning of homologs in various species, such as mouse, yeast and Drosophila. Not much information on the molecular basis of nucleotide excision repair in plants is available, but these organisms may have similar mechanisms to other eukaryotes. A homolog of XPB was isolated in Arabidopsis thaliana by using polymerase chain reaction (PCR) with degenerate oligonucleotides based on protein domains which are conserved among several species. Screening of an Arabidopsis cDNA library led to the identification and isolation of a cDNA clone with 2670 bp encoding a predicted protein of 767 amino acids, denoted araXPB. Genomic analysis indicated that this is a nuclear single copy gene in plant cells. Northern blot with the cDNA probe revealed a major transcript which migrated at approx. 2,800 b, in agreement with the size of the cDNA isolated. The araXPB protein shares approximately 50% identical and 70% conserved amino acids with the yeast and human homologs. The plant protein maintains all the f...Continue Reading

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Citations

Jan 17, 2004·Biochimie·Renata M A CostaCarlos F M Menck
Sep 21, 2000·Plant Science : an International Journal of Experimental Plant Biology·S KimuraK Sakaguchi
Jul 6, 2001·The Plant Journal : for Cell and Molecular Biology·Z LiuD W Mount
Dec 12, 2001·The Plant Journal : for Cell and Molecular Biology·R M CostaC F Menck
Jun 13, 2012·Journal of Biomedicine & Biotechnology·Claudia P Spampinato, Diego F Gomez-Casati
Aug 19, 2008·Mutation Research·Teresa Roldán-Arjona, Rafael R Ariza
Sep 13, 2003·The Plant Journal : for Cell and Molecular Biology·I-Peng ChenHolger Puchta
Feb 9, 2006·Chemical Reviews·Seisuke Kimura, Kengo Sakaguchi
May 11, 1999·Trends in Plant Science·A B Britt
Jan 13, 2005·Environmental and Molecular Mutagenesis·Bernard A KunzEdward J Vonarx
Aug 27, 2011·Biochimica Et Biophysica Acta·Anja MannussHolger Puchta

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