Cloning of a cellulase gene from the rumen anaerobe Fibrobacter succinogenes SD35 and partial characterization of the gene product

Letters in Applied Microbiology
N OzcanW J Harris

Abstract

A gene encoding an enzyme which degrades cellulose (end-1) was isolated from a library of Fibrobacter succinogenes SD35 DNA fragments and expressed in pUC18. The product of end-1 showed significant activity against carboxymethylcellulose but relatively minor activity against lichenan, xylan and avicel. The nucleotide sequence indicated a product of 388 amino acids with a molecular mass of 50.2 kDa. This was in agreement with the molecular size estimated by gel electrophoresis. No significant DNA sequence similarity was identified with any published endoglucanase.

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Citations

Oct 25, 2011·Applied Biochemistry and Biotechnology·Lei BaoHong Lu
Nov 26, 2003·FEMS Microbiology Reviews·Denis O KrauseChristopher S McSweeney
Mar 14, 1998·Molecular Pathology : MP·D Kipling, R G Faragher
Dec 24, 2018·Journal of Neuroscience Research·Suzhen ChenYonggui Yuan
Jun 1, 1997·Applied and Environmental Microbiology·S R MalburgC W Forsberg

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