PMID: 8605248Feb 7, 1996Paper

Cloning of a cluster of chitinase genes from Aeromonas sp. No. 10S-24

Biochimica Et Biophysica Acta
M ShiroM Arai

Abstract

A gene encoding chitinases from Aeromonas sp. No. 10S-24 was cloned into Escherichia coli DH5 alpha using pUC19, and its nucleotides were sequenced. The chitinase gene was clustered in ORFs (open reading frame) 1 to 4, in a 8-kb fragment of DNA. ORF-1 consisted of 1608 bp encoding 535 amino acid residues, and ORF-2 consisted of 1425 bp encoding 474 amino acid residues. ORF-3 was 1617 bp long and encodes a protein consisting of 538 amino acids. ORF-4 encodes 287 amino acids of the N-terminal region. The amino acid sequences of ORF-1 and ORF-3 share sequence homology with chitinase D from Bacillus circulans, and chitinase A and B from Streptomyces lividans. The amino acid sequence of ORF-2 shared sequence homology with chitinase II from Aeromonas sp. No. 10S-24, and chitinase from Saccharopolyspora erythraea. A region of the sequence starting from Ala-28 of the amino acid sequence of ORF-3 coincided with the N-terminal amino acid sequence of chitinase III from Aeromonas sp. No. 10S-24.

References

Jun 1, 1989·Journal of Biochemistry·K KameiT Ikenaka
Jun 1, 1983·European Journal of Biochemistry·G von Heijne
Nov 1, 1995·Bioscience, Biotechnology, and Biochemistry·M UedaM Arai
Jan 1, 1992·Bioscience, Biotechnology, and Biochemistry·M Veda, M Arai

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Citations

Mar 27, 2001·Enzyme and Microbial Technology·S -Y. WangN K. Singh
Oct 20, 2005·Journal of Bioscience and Bioengineering·K MorimotoK Ohmiya
Feb 7, 2003·Applied and Environmental Microbiology·Hiroshi TsujiboYoshihiko Inamori
Sep 15, 2015·Bioscience, Biotechnology, and Biochemistry·Kazushi SuzukiTakeshi Watanabe
Jan 1, 2009·Protein Expression and Purification·Yoon Gyo LeeHyeun-Jong Bae
Apr 1, 2014·Applied Biochemistry and Biotechnology·Jeen-Kuan ChenChao-Lin Liu
Jun 11, 1999·Bioscience, Biotechnology, and Biochemistry·A SaitoK Miyashita
Nov 17, 2006·Bioresource Technology·Yong-Seok LeeYong-Lark Choi
May 2, 2000·The Journal of Biological Chemistry·T IkegamiM Shirakawa
Nov 9, 2006·Applied Microbiology and Biotechnology·Kenji MorimotoKazuo Sakka
Nov 23, 2001·Canadian Journal of Microbiology·N Mabuchi, Y Araki
Mar 11, 2017·Applied Microbiology and Biotechnology·Shariza B JamekJørn D Mikkelsen
Jul 1, 1997·FEMS Microbiology Letters·J M PembertonR Schmidt
Jul 29, 2008·Biomacromolecules·Yoshihiro KikkawaTakeshi Tanaka
Oct 20, 2005·Journal of Bioscience and Bioengineering·K OhishiH Etoh

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