Cloning of a functional splice variant of L-type calcium channel beta 2 subunit from rat heart

The Journal of Biological Chemistry
Y YamadaN Tohse

Abstract

L-type Ca(2+) channels are heteromultimeric and finely tuned by auxiliary subunits in different tissues and regions. Among auxiliary subunits, beta subunit has been shown to play important roles in many functional aspects of Ca(2+) channel. Rat heart was reported to specifically express beta(2a) subunit. However, the slow inactivation rates of Ca(2+) currents recorded from recombinant Ca(2+) channels with the beta(2a) subunit, and the reported inability to detect beta(2a) subunit in rabbit heart by reverse transcription-PCR analysis raise the possibility of the existence of other beta subunits. We cloned a splice variant of beta(2) subunit from rat heart, using rapid amplification of cDNA 5' ends. The splice variant is highly similar to human beta(2c) subunit that was cloned from human ventricle. Northern blot analysis detected the rat beta(2c) subunit abundantly in rat heart and brain. The deduced amino acid sequence of the beta(2c) subunit was different from that of the beta(2a) subunit only in the N-terminal region. When the beta(2c) subunit was expressed along with alpha(1c) and alpha(2)delta subunits in baby hamster kidney cells, the inactivation rates were comparable with those from native cardiac myocytes, although those...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H L KimH Chin
Oct 1, 1982·Pflügers Archiv : European journal of physiology·G Isenberg, U Klockner
Jan 1, 1993·Annals of Neurology·M R RosenfeldH M Furneaux
Oct 1, 1995·Kidney International·E Perez-Reyes, T Schneider
Feb 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·H HaaseI Morano
Aug 11, 1997·Pflügers Archiv : European journal of physiology·D TrouetJ Eggermont
Mar 11, 1998·The Journal of Membrane Biology·A D Randall
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·N QinL Birnbaumer
Jun 24, 1998·Pflügers Archiv : European journal of physiology·T J Allen, G Mikala
Oct 3, 1998·Journal of Bioenergetics and Biomembranes·L BirnbaumerE Stefani

❮ Previous
Next ❯

Citations

Jul 8, 2010·Pflügers Archiv : European journal of physiology·Julio L AlvarezHannelore Haase
Aug 16, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Kai HuangYuhua Liao
Mar 23, 2004·European Journal of Pharmacology·Yasuhiro KamadaNoritsugu Tohse
Mar 11, 2004·Journal of Molecular and Cellular Cardiology·Po-Ju ChuPhilip M Best
Sep 12, 2003·Journal of Molecular and Cellular Cardiology·Takeshi KobayashiNoritsugu Tohse
Nov 5, 2002·Trends in Pharmacological Sciences·Steffen Hering
Sep 10, 2010·The Journal of Biological Chemistry·Vicenta Salvador-Recatalà, Robert M Greenberg
Jan 17, 2021·Life Sciences·Fumiaki NasuKuniaki Ishii
Feb 20, 2021·Journal of Pharmacological Sciences·Manabu MurakamiNoritsugu Tohse

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.