Cloning of a gene encoding a major secreted polypeptide of Listeria monocytogenes and its potential use as a species-specific probe.

Applied and Environmental Microbiology
R K FlammM F Thomashow

Abstract

A gene, designated msp, that encodes a major secreted polypeptide with a molecular mass of approximately 60 kilodaltons (kDa) was cloned from Listeria monocytogenes 10403. DNA hybridization analysis indicated that the msp gene was highly conserved among 15 independent L. monocytogenes isolates and that each of 5 isolates tested secreted a 60-kDa polypeptide that was immunologically related to the msp gene product. DNA sequences related to msp were not detected in any other Listeria species or in strains of Bacillus cereus, Bacillus thuringiensis, Streptococcus pyogenes, or Streptococcus pneumoniae when standard stringent DNA hybridization conditions were used. Under nonstringent conditions, related sequences were detected in Listeria ivanovii, Listeria seeligeri, and Listeria innocua, and immunoblot analysis indicated that these strains secreted polypeptides of about 60 kDa that were immunologically related to the msp gene product. The possibility of using the msp gene as a probe for the detection of L. monocytogenes and the potential functions of the msp gene product are discussed.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Sep 1, 1987·Applied and Environmental Microbiology·A R DattaW E Hill
Apr 1, 1988·The Journal of Experimental Medicine·D A PortnoyD J Hinrichs
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Manoil, J Beckwith
Jan 1, 1986·Infection and Immunity·J ParrisiusH P Seeliger
Feb 14, 1985·The New England Journal of Medicine·D W FlemingA L Reingold
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
Sep 1, 1984·Plasmid·P R Hirsch, J E Beringer
Nov 15, 1983·Journal of Molecular Biology·A M FrischaufN Murray
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Jul 1, 1963·Journal of Bacteriology·A N NJOKU-OBIV COVINGTON

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Citations

Jan 1, 1993·FEMS Microbiology Letters·R F WangM G Johnson
Sep 1, 1990·Letters in Applied Microbiology·P M BorderK W Siggens
Nov 1, 1995·International Journal of Food Microbiology·J E OlsenO Olsvik
Aug 1, 1991·Applied and Environmental Microbiology·A O Baloga, S K Harlander
Nov 1, 1996·Applied and Environmental Microbiology·R V Lachica
Dec 1, 1990·Applied and Environmental Microbiology·A R DattaJ Russell
Apr 1, 1991·Clinical Microbiology Reviews·A SchuchatC V Broome

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