PMID: 3754307Jan 1, 1986Paper

Cloning of a major developmentally regulated gene expressed in mature females of Schistosoma mansoni

Molecular and Biochemical Parasitology
A J Simpson, M Knight

Abstract

A cDNA library constructed from RNA isolated from adult Schistosoma mansoni has been screened by differential hybridization to identify clones corresponding to genes highly expressed by female worms. Several such cDNAs encoding the same highly abundant mRNA species were identified. Studies with one of these (pSF10) which contained a 500 base pair insert demonstrated that this gene was not expressed in immature females or eggs and encoded a polypeptide of approximately 35 000 daltons. Quantitation of the levels of RNA showed that 10% of the total RNA of female parasites was homologous to pSF10. A single gene corresponding to pSF10 was identified in Southern blotting experiments using adult worm DNA. The cloning of this gene will facilitate study of the molecular and genetic events controlling female schistosome maturation.

Citations

Apr 1, 1991·International Journal for Parasitology·M Kawanaka
Jul 1, 1987·Molecular and Biochemical Parasitology·B J ScallonC E Carter
Feb 1, 1989·Molecular and Biochemical Parasitology·T K WalkerA J Simpson
Jan 1, 1989·Molecular and Biochemical Parasitology·V RodriguesA J Simpson
Nov 1, 1991·Parasitology Today·P T Loverde, L Chen
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·F D RumjanekC Kelly
May 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J T Ribeiro-Paes, V Rodrigues
Jun 1, 1987·Diagnostic Microbiology and Infectious Disease·S E Maddison
Mar 25, 2000·International Journal for Parasitology·G R FrancoE M Rabelo
Jan 1, 1994·Parasitology·M R Fantappié, F D Rumjanek
Aug 21, 2009·Parasitology·Paul Hagan
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L BobekP T LoVerde
Aug 31, 2001·Experimental Parasitology·M R FantappiéF D Rumjanek
Aug 1, 2006·International Journal for Parasitology·Jennifer M Fitzpatrick, Karl F Hoffmann
Aug 16, 2011·Experimental Parasitology·Saskia deWalickJaap J van Hellemond
Jan 1, 1989·Experimental Parasitology·P T LoVerdeL A Bobek
Oct 1, 1991·Clinical Microbiology Reviews·S E Maddison

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.