Cloning of a mineral phosphate-solubilizing gene from Pseudomonas cepacia.

Applied and Environmental Microbiology
S Babu-KhanA H Goldstein

Abstract

We have recently shown that the ability of some gram-negative bacteria to dissolve poorly soluble calcium phosphates (Mps+ phenotype) is the result of periplasmic oxidation of glucose to gluconic acid via the quinoprotein glucose dehydrogenase (GDH), a component of the direct oxidation pathway. Escherichia coli K-12 derivatives synthesize apo-GDH but not the cofactor pyrroloquinoline-quinone (PQQ) essential for formation of the holoenzyme. Therefore, in the absence of exogenous PQQ, these strains do not produce gluconic acid and are Mps-. Evidence is presented to show that expression of a single 396-base Pseudomonas cepacia open reading frame (designated gabY) in E. coli JM109 (a K-12 derivative) was sufficient to induce the Mps+ phenotype and production of gluconic acid. We present the nucleotide sequence of this open reading frame which coded for a protein (GabY) with a deduced M(r) of 14,235. Coupled transcription-translation of a plasmid (pSLY4 or pGAB1) carrying gabY resulted in production of a protein with an M(r) of 14,750. Disruption of the open reading frame of gabY via site-directed mutagenesis changed the phenotype to Mps- and eliminated gluconic acid production. The deduced amino acid sequence of gabY has no apparen...Continue Reading

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Citations

Jul 20, 2002·FEMS Microbiology Letters·Fehmida FasimGeoffrey M Gadd
Oct 22, 2010·Canadian Journal of Microbiology·Manoj ShrivastavaS F D'Souza
Mar 6, 2015·Microbes and Environments·Hirohito TsurumaruKiwamu Minamisawa
Jun 9, 2018·Genome Announcements·Bang-Xiao ZhengKai Ding
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Jun 21, 2020·World Journal of Microbiology & Biotechnology·Burak AlaylarNaveen Kumar Arora
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Jan 9, 2019·Journal of Bacteriology·Maxwell R Fishman, Melanie J Filiatrault
Jan 17, 2019·Journal, Genetic Engineering & Biotechnology·Amit PandeSuresh Kaushik
Jul 6, 2021·Plant Physiology and Biochemistry : PPB·Gaurav RaturiRupesh Deshmukh

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