Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA

Biochemical and Biophysical Research Communications
T NakanishiM Takigawa

Abstract

Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Northern blot analysis showed that CTGF was highly expressed in HCS cells and rabbit growth cartilage cells in culture but was not expressed in osteoblastic cells in culture. In situ hybridization revealed that CTGF was expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. The expression of CTGF in HCS cells was up-regulated by the addition of TGF-beta or BMP-2. These findings suggest that CTGF participates in endochondral ossification.

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Citations

Apr 13, 2011·Journal of Cell Communication and Signaling·Satoshi Kubota, Masaharu Takigawa
Mar 14, 2002·The American Journal of Pathology·Maria Cristina ManaraKatia Scotlandi
Sep 21, 2001·The Biochemical Journal·N A WahabR M Mason
Aug 12, 2003·Journal of Neurotrauma·Brian D ClarkMary F Barbe
Feb 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroki Mori, Tohru Nakanishi
Oct 5, 2011·Critical Reviews in Eukaryotic Gene Expression·John A ArnottSteven N Popoff
Dec 10, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Diana S de JongWilma T Steegenga
Jan 13, 2006·Clinical and Experimental Pharmacology & Physiology·Janette K Burgess
Jul 3, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takashi NishidaMasaharu Takigawa
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jerome LafontCecile Martinerie
Jul 4, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Harumi KawakiMasaharu Takigawa
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May 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Carol A FernandoMatthew L Warman
Aug 27, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tadayoshi HayataMasaki Noda
Oct 2, 2009·Journal of Cellular Physiology·Kazumi KawataMasaharu Takigawa
Sep 16, 2003·Archives of Oral Biology·Manabu KanyamaMasaharu Takigawa
Aug 2, 2002·Biochemical and Biophysical Research Communications·Takanori EguchiMasaharu Takigawa
May 13, 2006·Biochemical and Biophysical Research Communications·Kazumi KawataMasaharu Takigawa
Dec 14, 2005·Biochemical and Biophysical Research Communications·Kazuki OmoteyamaMasaharu Sakai
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Sep 29, 2004·Osteoarthritis and Cartilage·Shunsuke OmotoMasaharu Takigawa
Oct 27, 2004·Osteoarthritis and Cartilage·J SaasT Aigner

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