Abstract
We isolated a novel lectin (Artocarpus nitidus subsp. lingnanensis lectin, ALL) from Artocarpus nitidus subsp. lingnanensis and showed its mitogenic activities. In this study, we determined the amino acid sequence of ALL by cDNA sequencing. ALL cDNA (933 bp) contains a 657-bp open reading frame (ORF), which encodes a protein with 218 amino acids. ALL shares high sequence similarities with Jacalin and Morniga G and belongs to jacalin-related lectin family. We also examined the antitumor activity of ALL using Raji, a human B-lymphoma cell line. ALL exhibits a strong binding affinity to cell membrane, which can be effectively inhibited by N-acetyl-D-galactosamine (GalNAc). ALL inhibits Raji cell proliferation in a time- and dose-dependent manner through apoptosis, evidenced by morphological changes, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Bcl-2 down-regulation, and caspase-3 activation. We further showed that the activation of p38 mitogen-activated protein kinase (MAPK) signaling pathways is required for the pro-apoptotic activity of ALL.
References
May 11, 2000·Plant & Cell Physiology·K HiranoD Hosokawa
Nov 25, 2000·Bioscience Reports·S S KomathM J Swamy
Dec 20, 2002·Fitoterapia·Euis Holisotan HakimHiromitsu Takayama
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 18, 2008·Cancer Letters·Bo LiuJin-ku Bao
Dec 23, 2008·Biochimica Et Biophysica Acta·Jure PohlevenJoze Brzin
Feb 6, 2009·Journal of Cell Science·Joslyn K Brunelle, Anthony Letai
Mar 11, 2009·Journal of Biomedical Science·Huan-Yao Lei, Chih-Peng Chang
Apr 30, 2009·Journal of Leukocyte Biology·Hervé BenoistPierre Rougé
Jan 26, 2010·Journal of Agricultural and Food Chemistry·Evandro Fei FangTzi Bun Ng
Mar 20, 2010·BMC Evolutionary Biology·Shu-Ye JiangSrinivasan Ramachandran
Jun 16, 2010·Journal of Structural Biology·Jingjin DingDa-Cheng Wang
Oct 5, 2010·Applied Microbiology and Biotechnology·Sze Kwan Lam, Tzi Bun Ng
Apr 15, 2011·PloS One·Nilanjana BanerjeeSampa Das
Aug 23, 2011·PloS One·Guillaume PoirouxHervé Benoist
Dec 2, 2011·Journal of Biomedicine & Biotechnology·Carla OliveiraLucília Domingues
Jun 2, 2012·Glycobiology·Shashikala R InamdarLu-Gang Yu
Nov 19, 2013·PloS One·Radha PujariPadma Shastry
Dec 18, 2013·Cancer Letters·Xinbing SuiHongming Pan
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams
Dec 3, 2014·ACS Chemical Biology·David MadariagaJesús M Peregrina
Jan 2, 2015·Frontiers in Bioscience (Landmark Edition)·Barbara GoliaGyula Timinszky
Jan 27, 2015·Acta Pharmacologica Sinica·Xin-xin XiongXiao-qian Chen
Feb 5, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Stephen Chu-Sung HuFeng-Lin Yen
Jan 23, 2017·Journal of Natural Medicines·Bo CuiJingping Wang
Feb 23, 2017·Frontiers in Pharmacology·Jayaram SaranyaSulochana Priya
Apr 21, 2017·Oncotarget·Ming-Horng TsaiChiang-Wen Lee