Cloning of a novel lectin from Artocarpus lingnanensis that induces apoptosis in human B-lymphoma cells

Bioscience, Biotechnology, and Biochemistry
Yu LuoQiyan Zeng

Abstract

We isolated a novel lectin (Artocarpus nitidus subsp. lingnanensis lectin, ALL) from Artocarpus nitidus subsp. lingnanensis and showed its mitogenic activities. In this study, we determined the amino acid sequence of ALL by cDNA sequencing. ALL cDNA (933 bp) contains a 657-bp open reading frame (ORF), which encodes a protein with 218 amino acids. ALL shares high sequence similarities with Jacalin and Morniga G and belongs to jacalin-related lectin family. We also examined the antitumor activity of ALL using Raji, a human B-lymphoma cell line. ALL exhibits a strong binding affinity to cell membrane, which can be effectively inhibited by N-acetyl-D-galactosamine (GalNAc). ALL inhibits Raji cell proliferation in a time- and dose-dependent manner through apoptosis, evidenced by morphological changes, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Bcl-2 down-regulation, and caspase-3 activation. We further showed that the activation of p38 mitogen-activated protein kinase (MAPK) signaling pathways is required for the pro-apoptotic activity of ALL.

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Citations

Apr 14, 2018·Journal of Natural Medicines·Linjie ZengLiejun Liao

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Datasets Mentioned

BETA
AAA32680.1
AAA32679.1
AAA3277.1
ALL09163.1
AAM90088.1
AAY35063.1
Y35064.1
AAL10685.1

Methods Mentioned

BETA
glycosylation
electrophoresis
PCR
confocal microscopy
reverse transcription-PCR
flow cytometry

Software Mentioned

DNAMAN
PSI
GraphPad Prism
MEGA4
ClustalX
ExPASy
BLASTn
SPSS
BLAST
BLASTp

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