PMID: 8589419Jan 1, 1996Paper

Cloning of a pectate lyase gene from Xanthomonas campestris pv. malvacearum and comparison of its sequence relationship with pel genes of soft-rot Erwinia and Pseudomonas

Molecular Plant-microbe Interactions : MPMI
C H LiaoL C Wong

Abstract

The cotton blight pathogen, Xanthomonas campestris pv. malvacearum strain B414, produces an extracellular pectate lyase (Pel) with an estimated M(r) of 41,000 and pI of 9.7. The gene coding for this enzyme initially identified in a 1.8-kb PstI genomic DNA fragment was cloned. The nucleotide sequences of this 1.8-kb fragment and two pel genes previously cloned from Pseudomonas fluorescens and P. viridiflava were determined. These pel genes encoded pre-Pel proteins consisting of 377 to 380 amino acids (a.a.). A signal peptide consisting of 26 to 29 a.a. was present at the amino-terminus of each pre-Pel. Multiple sequence analysis revealed that Pel proteins of non-Erwinia phytopathogens including Xanthomonas, Pseudomonas, and Bacillus constituted a distinct cluster, which showed 20 to 43% a.a. identity to the four established Pel families of Erwinia. Homologous pel sequences were detected in various pathovars or strains of X. campestris. All of these xanthomonads produced an alkaline Pel and were capable of causing soft-rot in potato tuber slices and green pepper fruits.

Citations

Oct 11, 2003·Biotechnology Advances·J W Chan, P H Goodwin
Feb 23, 2007·Molecular Plant-microbe Interactions : MPMI·Katrin JakobJoy Bergelson
Feb 1, 1992·Letters in Applied Microbiology·T W Wang, Y H Tseng
May 20, 2000·Biotechnology & Genetic Engineering Reviews·R A PradeA J Mort
Apr 10, 1999·Journal of Bacteriology·M A BekriA Vande Broek
Jul 16, 2009·Journal of Agricultural and Food Chemistry·Yi-Min HsiaoYi-Hsiung Tseng

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