Cloning of a putative sodium/calcium exchanger gene in the crayfish

Invertebrate Neuroscience : in
Bora Ergin, Nuhan Purali

Abstract

Crayfish is a common model animal for different experimental purposes. However, the lack of information about the genetic properties of the animal limits its use in comparison to other model animals. In the present study, a putative crayfish sodium/calcium exchanger gene has firstly been cloned in ganglia cDNA samples by conducting a series of PCR experiments, where a set of degenerate and specific primers and RACE method were used. The complete sequence is 2955 bp, and the ORF is 2718 bp in length. Molecular properties of the calculated peptide were similar to the sodium/calcium exchangers reported in the other species. Analysis of the qPCR data indicated that the putative gene has the highest expression level in the ganglia. However, an apparently elevated level of expression is observed in highly active tissues like heart, muscle and intestine, while the least expression level was observed in the stomach samples. It was proposed that the cloned gene may code the sodium/calcium exchanger protein in the crayfish.

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Citations

Sep 17, 2021·General and Comparative Endocrinology·Amanda C WeinerR Douglas Watson

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Datasets Mentioned

BETA
KT697616

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ClustalW2
Primer
BLAST
Blastn

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