Cloning of alpha 2u globulin cDNA using a high efficiency technique for the cloning of trace messenger RNAs

Gene
D T Kurtz, C F Nicodemus

Abstract

An extremely high-efficiency technique is described for cloning double-stranded (ds) cDNAs in Escherichia coli. The method, which uses two synthetic oligonucleotide linkers rather than one, results in approx. 200--500 recombinant clones per ng of ds cDNA. This technique was used to clone a cDNA comprising 95% of the full length of the mRNA of alpha 2u globulin, a male rat liver protein, which represents approx. 1% of hepatic messenger RNA. The cloned probe was applied to study the complex hormone controls of alpha 2u globulin mRNA in male and female rats.

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Citations

Apr 1, 1985·Biochemical Genetics·W A Held, J F Gallagher
Jul 1, 1996·Molecular and Cellular Endocrinology·D A Schwartz, D T Kurtz
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D M HelfmanS H Hughes
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S B Zimmerman, B H Pheiffer
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T MorinagaT Tamaoki
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R MokdadM Wegnez
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P S GuzelianJ A Gustafsson
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Sep 10, 1983·Nucleic Acids Research·A TsimanisE Gren
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Sep 1, 1983·Cell·E TaparowskyM Wigler
Mar 1, 1985·The American Journal of the Medical Sciences·J A Griffin
Sep 1, 1982·Journal of Cellular Physiology·L E Widman, L A Chasin
Mar 1, 1984·Journal of Virology·S S Monroe, S Schlesinger

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