Cloning of alpha-beta fusion gene from Clostridium perfringens and its expression

World Journal of Gastroenterology : WJG
Jia-Ning BaiBao-Hua Zhao

Abstract

To study the cloning of alpha-beta fusion gene from Clostridium perfringens and the immunogenicity of alpha-beta fusion expression. Cloning was accomplished after PCR amplification from strains NCTC64609 and C58-1 of the protective antigen genes of alpha-toxin and beta-toxin. The fragment of the gene was cloned using plasmid pZCPAB. This fragment coded for the gene with the stable expression of alpha-beta fusion gene binding. In order to verify the exact location of the alpha-beta fusion gene, domain plasmids were constructed. The two genes were fused into expression vector pBV221. The expressed alpha-beta fusion protein was identified by ELISA, SDS-PAGE, Western blotting and neutralization assay. The protective alpha-toxin gene (cpa906) and the beta-toxin gene (cpb930) were obtained. The recombinant plasmid pZCPAB carrying alpha-beta fusion gene was constructed and transformed into BL21(DE3). The recombinant strain BL21(DE3)(pZCPAB) was obtained. After the recombinant strain BL21(DE3)(pZCPAB) was induced by 42 degC, its expressed product was about 22.14% of total cellular protein at SDS-PAGE and thin-layer gel scanning analysis. Neutralization assay indicated that the antibody induced by immunization with alpha-beta fusion pro...Continue Reading

References

Jul 21, 1999·Viral Immunology·A M BennettR W Titball
Jan 11, 2003·British Journal of Pharmacology·Masahiro NagahamaJun Sakurai
Oct 22, 2003·Journal of Molecular Biology·Graeme C ClarkRichard W Titball
Jan 2, 2004·The Journal of Biological Chemistry·Sadayuki OchiJun Sakurai
Mar 9, 2004·Journal of Clinical Microbiology·Scott A SheedyRobert J Moore
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Marietta Flores-Díaz, Alberto Alape-Girón
Nov 2, 2004·Reviews of Physiology, Biochemistry and Pharmacology·J G SmedleyB A McClane
Dec 21, 2004·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·U SchotteH Neubauer

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