PMID: 8612643Mar 1, 1996Paper

Cloning of Arabidopsis thaliana glutathione synthetase (GSH2) by functional complementation of a yeast gsh2 mutant

European Journal of Biochemistry
P UllmannT J Bach

Abstract

Glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) plays an important role in the protection of plants against various types of stress caused by reactive oxygen species, gazeous pollutants, heavy metals and xenobiotics. A cDNA fragment containing the entire coding unit for glutathione synthetase (GSH2) of Arabidopsis thaliana was cloned by complementation of the methylglyoxal sensitivity of a gsh2 mutant of the yeast Saccharomyces cerevisiae. The cDNA encodes a protein of 478 amino acids (deduced Mr: 53783), bearing clear sequence similarities to GSH2 products from frog embryos (Xenopus laevis), rat kidney (Rattus norvegicus) and from the fission yeast (Schizosaccharomyces pombe). A highly conserved glycine-rich domain close to the carboxy-terminus was found in the GSH2 product and appears to be typical for eukaryotic glutathione synthetases. The Mr is similar to those of soluble animal enzymes, suggesting that the Arabidopsis gene also codes for a cytosolic protein. Genomic DNA-blot analysis indicates the presence of a single GSH2 gene. The yeast gsh2 mutant becomes resistant to methylglyoxal and cadmium after transformation with the plasmid bearing the Arabidopsis GSH2 cDNA. Moreover, this increased resistance is correlat...Continue Reading

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Citations

Dec 6, 2005·Photosynthesis Research·Philip M Mullineaux, Thomas Rausch
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·G Gutierrez-AlcalaL C Romero
Apr 16, 2009·The Journal of Biological Chemistry·Maika DeffieuNadine Camougrand
Nov 7, 2003·Molecular Plant-microbe Interactions : MPMI·Manuel A MatamorosManuel Becana
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Graham Noctor, Christine H. Foyer
Nov 10, 2011·The Plant Journal : for Cell and Molecular Biology·Varanavasiappan ShanmugamKuo-Chen Yeh
Jul 23, 2011·Plant, Cell & Environment·Graham NoctorChristine H Foyer
Feb 4, 2012·The Arabidopsis Book·Graham NoctorChristine H Foyer
Jun 13, 2006·The Journal of Biological Chemistry·Michael HothornKlaus Scheffzek
Feb 11, 2020·Frontiers in Plant Science·Eri AdamsRyoung Shin

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