PMID: 7010310Jan 10, 1981Paper

Cloning of bovine growth hormone gene and its expression in bacteria

Nucleic Acids Research
E KeshetH Aviv

Abstract

A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cloned DNA sequences are inserted into the beta-lactamase gene in the correct reading frame for BGH synthesis. The hybrid gene is expressed in bacteria and the product, a fused beta-lactamase-bovine growth hormone protein, is specifically immunoprecipitated with anti-serum to BGH. Unlike beta-lactamase, very little growth hormone containing sequences can be detected in the periplasmic space.

References

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Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K TalmadgeW Gilbert

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Citations

Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R FriesJ E Womack
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E De MaeyerK Atherton
Jun 1, 1983·The Proceedings of the Nutrition Society·I C Hart
Nov 25, 1982·Nucleic Acids Research·R P WoychikF M Rottman
Jan 1, 1984·Critical Reviews in Food Science and Nutrition·D Tusé
Jan 1, 1989·Animal Genetics·R FriesJ Womack
Sep 1, 1987·The Proceedings of the Nutrition Society·I C Hart

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