PMID: 2502398Jul 15, 1989Paper

Cloning of cDNA encoding the membrane-bound form of bovine beta 1,4-galactosyltransferase

European Journal of Biochemistry
G D'AgostaroM Tropak

Abstract

UDPgalactose: N-acetyl-D-glucosamine 4-beta-D-galactosyltransferase (EC 2.4.1.38) (GalT) is a Golgi-membrane-bound enzyme that participates in the biosynthesis of the oligosaccharide structures of glycoproteins and glycolipids. Synthetic DNA oligomers representing segments of the published partial cDNA sequence for bovine GalT were used as molecular probes to isolate from bovine-liver cDNA libraries overlapping cDNA clones that span 1728 nucleotides and potentially code for the entire polypeptide chain of bovine galactosyltransferase. The cDNA sequence for bovine GalT reveals a 1206-base-pair open reading frame that codes for 402 amino acids, including a presumptive N-terminal membrane anchoring domain of 20 hydrophobic amino acids. The colinearity between the cDNA sequence and 29 non-overlapping amino acid residues which were positively identified by N-terminal sequencing of two polypeptides isolated from the soluble form of the enzyme was consistent with the translation frame and confirmed the authenticity of the cDNA clones. The finding of an N-terminal hydrophobic segment which serves as the membrane anchor and signal sequence suggests that the C-terminal region of the GalT polypeptide is oriented within the lumen of the Go...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1977·The Journal of Cell Biology·J T PowellK Brew
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N L ShaperG F Hollis
Mar 22, 1985·Science·D J Lipman, W R Pearson
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H NarimatsuP K Qasba
Jul 16, 1986·Biochemical and Biophysical Research Communications·H E AppertD J McCorquodale
Aug 29, 1986·Biochemical and Biophysical Research Communications·H E AppertD J McCorquodale
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M G Humphreys-BeherV J Kidd
Nov 1, 1986·Molecular and Cellular Biochemistry·E M BaynaB D Shur
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A R WymanD Botstein
Oct 25, 1985·Science·W T Wickner, H F Lodish
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Feb 1, 1982·Molecular and Cellular Biology·H Okayama, P Berg
May 5, 1983·Journal of Molecular Biology·J A Gough, N E Murray
Nov 18, 1983·Science·R A Young, R W Davis
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W J Wilbur, D J Lipman
Jun 1, 1983·European Journal of Biochemistry·G von Heijne
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

❮ Previous
Next ❯

Citations

Nov 24, 1999·Glycobiology·S Kitazume-KawaguchiK J Colley
Mar 14, 1991·European Journal of Biochemistry·K NakazawaH Narimatsu
Dec 1, 1995·Glycoconjugate Journal·E E BoeggemanP K Qasba
Sep 26, 2002·Journal of Biochemistry and Molecular Biology·Jae Eun ParkSang Soo Lee
May 23, 1997·The Journal of Biological Chemistry·S K Cho, R D Cummings
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·T SatoI Van Die
Jan 1, 1993·Critical Reviews in Clinical Laboratory Sciences·I Brockhausen
May 17, 2000·FEBS Letters·M OubihiT Matsuda
Jul 18, 2002·Glycobiology·Elizabeth Boeggeman, Pradman K Qasba
Jun 24, 2004·The Journal of Membrane Biology·W W Young
Mar 9, 2002·Biochemical and Biophysical Research Communications·Boopathy RamakrishnanPradman K Qasba
Dec 21, 1993·Biochimica Et Biophysica Acta·R Kleene, E G Berger
Oct 1, 1993·Molecular and Cellular Biology·P SinghW Hong
Feb 1, 1996·Chemical Reviews·Harrie J. M. GijsenChi-Huey Wong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.