Cloning of cDNA sequences encoding cowpea (Vigna unguiculata) vicilins: Computational simulations suggest a binding mode of cowpea vicilins to chitin oligomers

International Journal of Biological Macromolecules
Antônio J RochaThalles Barbosa Grangeiro

Abstract

Vicilins are 7S globulins which constitute the major seed storage proteins in leguminous species. Variant vicilins showing differential binding affinities for chitin have been implicated in the resistance and susceptibility of cowpea to the bruchid Callosobruchus maculatus. These proteins are members of the cupin superfamily, which includes a wide variety of enzymes and non-catalytic seed storage proteins. The cupin fold does not share similarity with any known chitin-biding domain. Therefore, it is poorly understood how these storage proteins bind to chitin. In this work, partial cDNA sequences encoding β-vignin, the major component of cowpea vicilins, were obtained from developing seeds. Three-dimensional molecular models of β-vignin showed the characteristic cupin fold and computational simulations revealed that each vicilin trimer contained 3 chitin-binding sites. Interaction models showed that chito-oligosaccharides bound to β-vignin were stabilized mainly by hydrogen bonds, a common structural feature of typical carbohydrate-binding proteins. Furthermore, many of the residues involved in the chitin-binding sites of β-vignin are conserved in other 7S globulins. These results support previous experimental evidences on the a...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1986·Annual Review of Biochemistry·F A Quiocho
Nov 1, 1995·Proteins·A SaliM Karplus
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Sep 28, 1999·Bioinformatics·A BohneC W von der Lieth
Jan 11, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M P SalesJ Xavier-Filho
May 29, 2001·Archives of Insect Biochemistry and Physiology·W R Terra
Dec 31, 2003·Phytochemistry·Jim M DunwellSawsan Khuri
Mar 20, 2004·Journal of Agricultural and Food Chemistry·Regina L FreitasRicardo B Ferreira
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Jan 16, 2008·Journal of Agricultural and Food Chemistry·Leonardo L P MacedoMaurício P de Sales
Apr 25, 2009·Acta Crystallographica. Section D, Biological Crystallography·Charles Simon Bond, Alexander Wolfgang Schüttelkopf
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Sep 17, 2011·Journal of Chemical Information and Modeling·Roman A Laskowski, Mark B Swindells
Nov 30, 2011·Journal of Chemical Theory and Computation·Olgun GuvenchAlexander D Mackerell
Jun 4, 2013·BMC Biotechnology·Marina Duarte Pinto LoboThalles Barbosa Grangeiro
Jun 6, 2013·Nucleic Acids Research·Lim HeoChaok Seok
Nov 14, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gabriel B OliveiraCarlos P Silva
Jun 21, 2017·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Daniele KunzCarlos P Silva
Jan 18, 2018·Bioscience, Biotechnology, and Biochemistry·Ederlan de Souza FerreiraAlessio Scarafoni

❮ Previous
Next ❯

Citations

Feb 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Faiza RasheedEva Johansson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
M P SalesJosé Xavier-Filho
Journal of Agricultural and Food Chemistry
Adriana Ferreira UchôaJosé Xavier-Filho
Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
M P SalesJosé Xavier-Filho
Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Maria Ligia R MacedoJosé Xavier-Filho
© 2022 Meta ULC. All rights reserved