PMID: 3753797Jan 24, 1986Paper

Cloning of cDNA sequences of a progestin-regulated mRNA from MCF7 human breast cancer cells

Nucleic Acids Research
D ChalbosH Rochefort

Abstract

A cDNA clone corresponding to an mRNA regulated by the progestin R5020, has been isolated by differential screening of a cDNA library from the MCF7 breast cancer cell line, which contains estrogen and progesterone receptors. This probe hybridized with a single species of poly A + RNA of 8-kb molecular weight as shown by Northern blot analysis and could also be used to total RNA preparation. This recombinant clone hybridized specifically to an mRNA coding for a 250,000 daltons protein when translated in vitro. This protein was identical to the 250 kDa progestin-regulated protein that we previously described (Biochem. Biophys. Res. Commun. 121, 421-427, 1984) as shown by immunoprecipitation with specific rabbit polyclonal antibodies. Dose-response curve and specificity studies show that the accumulation of the Pg8 mRNA and that of the 250-kDa protein was increased by 5 to 30-fold following progestin treatment and that this effect was mediated by the progesterone receptor. Time course of induction indicated that the accumulation of mRNA was rapid and preceded that of the protein. This is the first report on a cloned cDNA probe of progestin-regulated mRNA in human cell lines.

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Citations

Jan 1, 1990·The International Journal of Biochemistry·J F SavouretE Milgrom
May 1, 1988·Journal of Steroid Biochemistry·E BadiaJ C Nicolas
Mar 1, 1989·Journal of Steroid Biochemistry·J F Glover, P D Darbre
Jun 1, 1994·Molecular and Cellular Endocrinology·E KalkhovenB van der Burg
Sep 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·D ChalbosH Rochefort
Jan 1, 1996·Breast Cancer Research and Treatment·M J PilatS C Brooks
Nov 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·R B DicksonM E Lippman
Jan 1, 1989·Journal of Steroid Biochemistry·M E Lippman, R B Dickson
Jul 1, 1989·Journal of Steroid Biochemistry·L R WisemanB R Westley
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Nov 17, 1994·Biochimica Et Biophysica Acta·L W GonzalesJ Gonzales
Jan 1, 1990·Annals of the New York Academy of Sciences·D ChalbosH Rochefort
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R E HallR L Sutherland
Aug 1, 1997·Endocrine Reviews·J D Graham, C L Clarke
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sara J PrestBruce R Westley
Mar 15, 1996·The Journal of Biological Chemistry·E KalkhovenB van der Burg
Jul 11, 2009·Endocrinology·Naeem AmiryPeter E Lobie
Aug 18, 2004·Cancer Research·Aurélie BardinGwendal Lazennec

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