Cloning of chemically synthesized lactose operators

Gene
J R SadlerM H Caruthers

Abstract

Recombinant DNA molecules, constructed from the ColE1-Mk5 hybrid plasmid PMB9 and a chemically synthesized wild-type lactose operator segment, have been used to transform Escherichia coli. Up to 10% of the transformants (selected for the tetracycline-resistance property of PMB9) are partially constitutive for the lactose operon enzyme beta-galactosidase. In vitro studies demonstrate that these partially constitutive transformants contain plasmid DNA molecules which carry one or more lactose operators, and which will bind purified lactose repressor. Preliminary results with some modified operator sequences are also presented.

References

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Citations

Jul 1, 1978·Gene·D L Hare, J R Sadler
Jan 1, 1981·Gene·J R Sadler, M Tecklenburg
May 1, 1984·Gene·A H KoopS Bourgeois
Oct 30, 1989·Gene·D S Bautista, F L Graham
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Aug 26, 1985·Nucleic Acids Research·D Vidal-Ingigliardi, O Raibaud
Aug 25, 1980·Nucleic Acids Research·G Herrick
Oct 1, 1980·Journal of Virology·A HorwichW Eckhart

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