Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region

Biochemical and Biophysical Research Communications
Hongwei RenMingming Chu

Abstract

The genomic DNA of crucian carp (Carassius cuvieri) metallothionein-II (ccMT-II), with its upstream region, was obtained. The sequence analysis of its upstream region revealed several putative cis-acting elements including seven metal regulatory elements (MREs), three activator protein 1 (AP1), two glucocorticoid response elements (GREs), etc. The seven MREs locate into two clusters, a distal cluster with four MREs within -800/-600bp from the translation start site and a proximal cluster with three MREs close to TATA box. In transient luciferase gene expression assays, both of the distal and proximal cluster MREs have significantly shown synergistic effects in the transcription of ccMT-II gene; the proximal cluster of MREs serves as the major elements in metal inducing activity; Zn(2+) and Cd(2+) served as much stronger inducers than Cu(2+) shown in ccMT-II expression. The two GRE homologous sequences in ccMT-II promoter showed not to be inductive in either HepG2 or HEK293.

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J J Schroeder, R J Cousins
Aug 11, 1990·Nucleic Acids Research·M F MurphyB Howard
Sep 1, 1989·Gene·L P Fernando, G K Andrews
Jan 1, 1987·Experientia. Supplementum·R D Palmiter
Nov 15, 1988·Biochemistry·J H Kägi, A Schäffer
Oct 1, 1985·Molecular and Cellular Biology·M KarinG Wong
Jan 1, 1982·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·H KitoT Tazawa
Jan 1, 1984·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M Roch, J A McCarter
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·A W Senear, R D Palmiter
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Oct 20, 1995·The Journal of Biological Chemistry·S L SamsonL Gedamu
Nov 30, 1994·Biochemical and Biophysical Research Communications·K M Chan
Feb 1, 1996·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·S G GeorgeJ Wright
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·E J KellyR D Palmiter
Jan 15, 1998·Progress in Nucleic Acid Research and Molecular Biology·S L Samson, L Gedamu
Apr 6, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·N MutoK Tanaka
Oct 29, 2000·Progress in Nucleic Acid Research and Molecular Biology·K Ghoshal, S T Jacob
Mar 20, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·E HermeszJ Nemcsók
Feb 13, 2003·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Yohan MourgaudJean-Claude Amiard
Jan 30, 2004·Biochimica Et Biophysica Acta·Pat Chun ChanKing Ming Chan
Jul 13, 2004·Biochimica Et Biophysica Acta·Carol Hiu Mei Yan, King Ming Chan

❮ Previous
Next ❯

Citations

Sep 14, 2010·Ecotoxicology and Environmental Safety·Halina I FalfushynskaYoon Kwon Nam
Jun 28, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Dian GaoPin Nie
Jun 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·T D SingerP M Schulte
Mar 6, 2007·The International Journal of Biochemistry & Cell Biology·Pengfei HeHongwei Ren
Dec 14, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nic R BuryChrister Hogstrand
Sep 25, 2007·Ecotoxicology and Environmental Safety·Young Sun ChoYoon Kwon Nam

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.