Cloning of mouse DAN cDNA and its down-regulation in transformed cells

Japanese Journal of Cancer Research : Gann
T OzakiS Sakiyama

Abstract

Recently, we have demonstrated that DAN gene product exhibits a tumor-suppressive activity in vitro. We report here the cloning and sequencing of a mouse DAN cDNA that contains the entire coding region. Sequence analysis revealed that mouse DAM cDNA is 1691 nucleotides in length and contains an open reading frame of 178 amino acids. The deduced mouse DAN protein sequence shows 96% and 93% identity with the counterparts isolated from rat 3Y1 fibroblasts and normal human lung, respectively. Genomic Southern blot hybridization indicated that DAN gene exists as a single copy in the mouse genome. The expression of DAN gene was suppressed in a variety of transformed NIH3T3 cells when compared with that in the parental NIH3T3 cells.

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Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T Ozaki, S Sakiyama

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Citations

Mar 28, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y NakamuraS Sakiyama
Sep 10, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Mohammad Zeeshan OzairAli H Brivanlou
Dec 4, 2001·Mechanisms of Development·J OgitaH Koseki
Nov 30, 1998·Mechanisms of Development·E StanleyR P Harvey
Apr 20, 1999·Developmental Biology·J J PearceJ Rossant
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lisa M Gerlach-BankKate F Barald
May 2, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lisa M Gerlach-BankKate F Barald
Nov 5, 1997·DNA and Cell Biology·T OzakiS Sakiyama
Jul 2, 1999·DNA and Cell Biology·T OzakiS Sakiyama

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